PLANT BREEDING AND SEED PRODUCTION OF AGRICULTURAL CROPS
In the conditions of the Ryazan region there have been studied 64 spring common wheat varieties from the collection of the Federal Research Center VIGRR named after Vavilov in order to identify valuable traits for use in breeding. Field trials were carried out in the collection nursery on dark gray forest heavy loamy soil in 2018–2022, using the methodological recommendations of the Federal Research Center VIGRR named after Vavilov. There has been established that the highest yields (more than 5.0 t/ha) were formed by the varieties ‘Arseya’, ‘Maestro’ (Ryazan region), ‘KWS Akvilon’, ‘Ethos’ (Germany), ‘KWS Torridon’ (Great Britain), ‘Odeta’ (Czech Republic), ‘Calispero’ (France), which was on 29.9–49.9 % higher than the mean variety productivity in the trial. Yield stability with the least variation (Cv) was established for the varieties from the Central region – RIMA, ‘Arseya’ (Ryazan region), ‘Zlata’ (Moscow region) and ‘Omskaya 36’ (West Siberian region) with 3.1–9.6 %. The studied assortment of plant heights was divided into groups, such as dwarfs (< 60 cm) – 4.7 %, semi-dwarfs – 12.5 %, medium-sized – 75.0 %, tall – 7.8 %. There were identified 8 early ripening varieties ‘Chelyaba 2’ (Chelyabinsk region), ‘Odeta’ (Czech Republic), ‘Zlata’ (Moscow region), ‘Novosibirskaya 29’, ‘Novosibirskaya 15’, ‘Polyushka’ (Novosibirsk region), ‘M-83-1531’ (USA), ‘Burlak’ (Ulyanovsk region). The varieties ‘Voevoda’ and ‘Favorit’ (Saratov region) showed high resistance to various pathogens. There has been found a strong correlation between productivity and the density of productive stems with r = 0.753±0.084. 0.808±0.075, an average correlation with a number of grains per head with r = 0.427±0.115. 0.716±0.089 and grain weight per head with r = 0.374±0.118...0.689±0.092. Grain weight per head was largely determined by a number of grains per head with r = 0.621±0.099. 0.824±0.072.
The current paper has presented the characteristics of the new pea variety ‘Antey’ of Altai breeding according to the most important economically valuable and morphological characteristics. Breeding work to develop a variety at the FSBSI “Federal Altai Research Center of Agrobiotechnologies” began in 2003 from the moment of hybridization of the original parental forms. The variety ‘Antey’ was developed by individual selection from a hybrid combination (Varyag x Novosibirets) x Batrak. The species of the variety was ecaducum. On average, over the years of testing (2020–2022), the variety ‘Antey’ reliably differed from the standard variety ‘Batrak’ in terms of a set of breeding valuable traits. This variety has the most important economic and biological indicators, such as high productivity (2.75 t/ha), resistance to lodging (5 points), low susceptibility to diseases (4.5 points), high protein percentage (23.5 %). Based on phenological observations of pea crops, there have been identified some morphological features of this variety. The variety ‘Antey’ is a middle maturing one. The vegetation period lasts 70...75 days. The variety is determinate, plant height is 65 cm. The leafless type and compressed internodes of the new pea variety increase the resistance of shoots to lodging. The plants bear white flowers of medium size. Beans are shelled, straight. The number of seeds in a bean is 4–7. Our research has shown that over the course of three years in the Competitive Variety Testing, according to the above-mentioned traits, the variety ‘Antey’ significantly exceeds those of the standard variety ‘Batrak’, which made it possible to send this pea variety to the State Variety Testing in 2022. The variety ‘Antey’ is going to be tested in region 10 (West Siberian).
The aim of the work is to analyze the physical and mechanical properties of grain of spring barley varieties cultivated in the conditions of the Predkamsk zone of the Republic of Tatarstan. The research was conducted in Tatarsky Research Institute of Agriculture FRC of Kazan Research Center RAS in 2015–2022. In the conditions of the Predkamsk zone, on average over the years of research, the late maturing variety Endan was characterized by reliably high values of 1000 grain weight (48.5 g), the early maturing variety Kamashevsky – reliably high values of natural grain weight (686, 3 g/l), convergence from 2.8 x 20 mm sieve (33.9 %) and coarseness (70.02 %), medium maturing variety Raushan – higher variability in 1000 grain weight (18.6 %), convergence from 2.8 and 2.5 x 20 mm sieves (55.6 and 29.7 %, respectively) and coarseness (39.5 %). The direct dependence of the mass of 1000 grains, runoff from the sieve 2.8 x 20 mm and coarseness on the sum of precipitation (r = 0.41/0.58/0.56, respectively), GTC (r = 0.40/0.58/0.55, respectively) and inverse dependence of these physical and mechanical properties on the average daily air temperature (r = -0.51/-0.62/-0.63, respectively) of the interphase period "earing-full ripeness" were established at 1% significance level. Judging by the value of the coefficient of determination (r2), the most closely related to the average daily air temperature are the signs "mass of 1000 grains", "drift from the sieve 2.8 x 20 mm" and "coarseness". The analysis of path coefficients revealed a high direct positive contribution (0.87) of 2.8 x 20 mm sieve runoff and an average direct positive contribution (0.43) of 2.5 x 20 mm sieve runoff to grain yield. Under the conditions of the Predkamsk zone, the prevailing contribution to the total variance of the traits "drift from the sieve 2.8 x 20 mm", "coarseness", "weight of 1000 grains", "bulk grain weight", "drift from the sieve 2.5 x 20 mm" was made by the factor "year", which accounted for (in descending order) 81.7...80.3...76.6...76.2. 68.7 %, respectively.
Hybridization is the main method for developing new breeding material, obtaining genetic variability, and combining desired traits. The purpose of the current work is to characterize new promising varieties and hybrids of sweet and grass sorghum developed by the “ARC “Donskoy” because of hybridization on a sterile and fertile basis. The study was conducted on the breeding field of the FSBSI “ARC “Donskoy”, Zernograd, Rostov region in 2021–2023. The zone is characterized by ordinary carbonate chernozem (3.2 % of humus, 10–40 mg/kg of phosphorus, 70–110 mg/kg of nitrogen, 300–500 mg per kg of soil of potassium). Meteorological conditions during the years of study (2021–2023) were contrasting: in 2022, HTC = 0.52 indicated average aridity and insufficient moisture with 0.81–0.82 in 2021 and 2023. The objects of the study were the varieties and hybrids of Sudan grass and sugar sorghum developed by the FSBSI “ARC “Donskoy”. As a result of hybridization on a sterile basis, there was developed a number of sorghumSudan hybrids (‘Jetta x Kudesnitsa’, ‘APV-1115 x Topolina’, ‘APV-1115 x FP’, ‘Dobrynya’, ‘Gordey’) and sweet sorghum hybrids (‘APV-115 x Yuzhnoye’, ‘Jetta x Severanay Vishnya’, ‘Duet’), allowing to obtain green mass yields of 51–63 and 47–49 t/ha, which was higher than the standard by 5–17 t/ha (10.8–37.0 %) and 5–7 t/ha (11.9–16.7 %) respectively. Using intervarietal hybridization, almost all varieties developed by the FSBSI “ARC “Donskoy” have been created. Among them there are the Sudan grass varieties ‘Alisa’, ‘Gratsiya’, ‘Anastasiya’, ‘Kudesnitsa’, ‘FP’ and the sweet sorghum varieties ‘Yuzhnoe’, ‘Feniks’, ‘Orfey’, ‘SK-20/334’, ‘Severanay Vishnya’ with a green mass yield of 40–48 and 34–38 t/ha, which is higher than that of standard varieties by 3–11 t/ha (8.1–29.7 %) and 7–11 t/ha (25.9–40.7 %), respectively. Thus, hybridization is the main method for developing new breeding material and combining the desired traits in new samples.
There have been given the study results of grain color characteristics of winter durum wheat varieties and lines (Triticum durum Desf.). There has been studied the effect of factors (‘variety’, ‘year’ and their correlation) on the expression of the main breeding-valuable traits that affect the quality of the final product. The purpose of the current study was to determine the variability of phenotypic characteristics of grain associated with the number of yellow pigments in grain, the color of semolina and finished pasta, to study the correlation between indicators and to identify the most promising ones for further work. Field trials were carried out in the scientific crop rotation laboratory of breeding and seed production of winter durum wheat of the FSBSI “ARC “Donskoy”. Laboratory and analytical study was carried out in the laboratory of biochemical, technological and agrochemical estimation in accordance with generally accepted methods and GOST. As a result of the study, there has been established that the main effect on such characteristics of grain quality of winter durum wheat as ‘general hardiness’, ‘mass fraction of protein in grain’ and ‘grain color index b’ was produced by the factor ‘year of study’ by 67.0 %, 48 .2 % and 65.4 % respectively. The ‘cereal color index b’, ‘dry pasta color index b’ and visual estimation of dry pasta were mainly influenced by the factor ‘genotype’ by 62.3 %, 82.5 % and 48.3 %, respectively. The severity of the trait ‘content of carotenoid pigments’ depended on all factors. Over the years of the study, the winter durum wheat varieties and lines developed by the FSBSI “ARC “Donskoy” according to color characteristics met the international requirements for durum wheat grain. As a result, the varieties ‘Grafit’ and ‘Karotinka’ stood out according to several studied characteristics in connection with which we recommend expanding the production crops of these varieties. There can be recommended to use promising lines 1147/19 and 971/19 and the variety ‘Solntsedar’ in the breeding process as sources with high color characteristics of grain and pasta.
Work on developing a new pea variety ‘Krasnoufimsky 20’ began in 2008 on the experimental fields of the Krasnoufimsky breeding center of the Ural Research Institute of Agriculture, a branch of the FSBSI UrFARC UrO RAS. The purpose of the current study was to develop a highly productive pea variety that is resistant to negative environmental conditions, resistant to diseases and pests, and has a non-lodging stem and non-shedding seeds. The greatest productivity excess of the variety ‘Krasnoufimsky 20’ compared to the standard was observed in 2017, 2019, 2021 and 2022 (from 11 % to 62 %). On average, ‘Krasnoufimsky 20’ gave a significant excess over the standard by 0.26 t/ha. The variety ‘Krasnoufimsky 20’ requires weather conditions in the HTC range of 1.0–2.1 to obtain stable productivity. But regardless of environmental factors, both in favorable conditions and during periods of waterlogging or drought, the variety ‘Krasnoufimsky 20’ exceeded the standard by 0.03–0.83 t/ha. The variety ‘Krasnoufimsky 20’ ripened 1–3 days earlier than the standard, it has a larger number of beans and seeds per plant (by 0.2 and 1.5 pieces, respectively). The variety ‘Krasnoufimsky 20’ has a high seed productivity per plant (2.3 g). The variety is affected with ascochyta blight and root rot at the standard level, its damage by the codling moth is less than the standard. The new variety ‘Krasnoufimsky 20’ exceeded the standard variety Krasnoufimsky 11 and other varieties of local breeding according to adaptability. In 2023, it was decided to include the new pea variety ‘Krasnoufimsky 20’ in the State List of Breeding Achievements approved for use in the regions of Volga-Vyatka, North Caucasus, Ural, West Siberian.
The development and wide distribution of new adaptive varieties in production is a promising and environmentally friendly way for the development of agriculture. A new, more modern, highly productive variety can ensure increased productivity, resistance of crops to stress factors, and promotes better use of natural and anthropogenic resources. The leading direction in breeding in the nearest future should be not the development of varieties in general, adapted to growing conditions, but the identification of varieties adapted to the stress-limiting environmental factors of a particular region, adaptive to the challenges of nature. In addition to the characteristics of the variety and environmental conditions, the forecrop also influences its productivity. Selection in some conditions may not reveal the potential of the genotype in others, which makes it important to obtain information on different growing environments of varieties at the early stages of breeding. The purpose of the current study was to determine the adaptive properties of promising winter barley varieties and lines according to various forecrops. The study was carried out from 2021 to 2023 to estimate the adaptability parameters of 20 winter barley varieties and lines developed by the FSBSI Agricultural Research Center “Donskoy” for various forecrops (weedfree fallow, peas, sunflower, winter wheat). To determine the indicators of homeostaticity (Hom) and selection value (Sc), stress resistance (Ymin–Ymax) and genetic flexibility ((Ymax+Ymin)/2), the coefficient of responsiveness to favorable growing conditions (Kr) and the indicator of the stability level of the variety (VSLI), there have been applied the methods of V.V. Khangildin and N.A. Litvinenko (1981), A.A. Rosielle and J. Hamblina as presented by A.A. Goncharenko (2005), V.A. Zykin (2005), E.D. Nettevich (2001). According to all forecrops, there have been identified the varieties distinguished by high parameters of environmental adaptability and stability. Stress-resistant genotypes included the lines ‘Parallelum 2016’ and ‘Parallelum 2017’ (Ymin–Ymax = 1.2, 1.1, respectively), which were distinguished by a set of stability indicators and have been recommended for use in the breeding process as sources of high stress resistance, low yield variability, capable of minimizing negative impact of growing conditions. The variety ‘Step’ proved to be adaptable (regression coefficient bi = 1.5), genetically flexible ((Ymin–Ymax)/2 = 7.7), and highly responsive to growing conditions (Kr = 1.53). The dynamics of productivity corresponded to changes in growing conditions, and to maximize a productivity level, there has been recommended to cultivate it on intensive forecrops.
The current study was carried out on the experimental fields of SES, a branch of VNIIMK in 2021–2023. The purpose was to estimate spring rape varieties and hybrids of various ecological and geographical origins based on the main economically valuable traits, to identify the most productive and adapted to the conditions of Western Siberia. The objects of the study were 16 varieties and hybrids of domestic and foreign breeding. The variety ‘Yubileiny’ and the hybrid ‘Ozorno’ were used as a standard. There was established that environmental testing of spring rape varieties and hybrids made it possible to identify significant differences in the main economically valuable traits. In the conditions of Western Siberia, among the rape varieties, the maximum productivity over three years of study was demonstrated by the varieties ‘Yarky’ (2.50 t/ha), ‘Sibiryak 60’ (2.43 t/ha) and ‘55region’ (2.42 t/ha), and among the hybrids ‘PR46X75’ (2.68 t/ha) and ‘Salsa KL’ (2.57 t/ha) were the best according to this indicator. The highest seed productivity was obtained in 2021 and ranged from 2.51 to 3.29 t/ha for varieties and from 3.02 to 3.52 t/ha for hybrids. High oil content in seeds was noted under the conditions of 2022 in such varieties as ‘Granit’, ‘55region’, ‘Yarkiy’, ‘Amulet’, ‘Geros’ and amounted to 51.2–52.6 %. With a short vegetation period of up to 85 days, the varieties ‘Amulet’, ‘Ruyan’, ‘Erebus’ and ‘Forpost KL’ were identified as early maturing varieties. Middle maturing varieties with a vegetation period of 88–90 days included ‘Granit’, ‘Yarkiy’, ‘Prometey’ and ‘Geros’. The plant height varied from 80 to 101 cm for hybrids and from 88 to 120 cm for rape varieties. The trait ‘1000-seed weight’ varied from 3.6 to 4.2 g. The varieties ‘55region’, ‘Sibiryak 60’, ‘Yarkiy’, ‘Amulet’, ‘Prometey’ and ‘Forpost KL’ were of greatest interest for practical breeding according to the complex of economically valuable traits in the southern forest-steppe of Western Siberia.
The current paper has provided data on the study of the vascular system of flag leaves of rice plants grown in a greenhouse in the period 2022–2023. The objects of the study were 24 rice samples developed at the FSBSI “ARC “Donskoy” using the method of androgenesis and cell culture. The purpose of the work was to determine the degree of development of the vascular system of flag leaves of regenerated rice plants with different ploidy levels (1n, 2n, 4n). When studying the vascular system of plant leaves (the number and area of bundles), it was found that haploid plants had smaller vascular bundles compared to diand tetraploid samples. The average beam diameter had dimensions of 59.5, 69.3 and 75.3 µm, the area of one beam was 2815.6, 3827.2 and 4540.5 µm², respectively. In the leaves of rice samples, a larger number of small and large vascular bundles have been formed with increasing ploidy level. Their number was 36–44 in haploids, 40–52in diploids, 52–60 in tetraploids. The average number of bundles was 40.3, 46.6 and 55.2, respectively. The venation pattern was individual for each sample. Between single large bundles there are from one to seven small veins, most often 4–6 pieces. Thus, samples with different ploidy levels differ in the anatomical structure of the leaves, which ultimately affects their morphology and productivity.
GENERAL AGRICULTURE AND PLANT BREEDING
The purpose of the current study was to identify the optimized seeding rate of the spring barley variety ‘Lyuboyar’ to obtain an economically profitable yield in the conditions of the non-blackearth part of the Central region of the Russian Federation on dark gray forest soils of a mean fertility level. The trials were carried out in the conditions of the Ryazan region in the selection crop rotation when sown in “black” fallow in 2022–2023. The trial scheme included the study of 6 options: 3.0; 3.5; 4.0; 4.5; 5.0 and 5.5 million germinating seeds per 1 hectare. There has been established that the optimal option in terms of field germination and preservation of plants for harvesting is the norm of 4.0 million per 1 ha with 89.5 % and 77.5 %, respectively. The number of survived plants before harvesting, the number of productive stems, plant height, head length, number of grains per head and grain weight per head have a significant contribution to crop productivity (r = +0.727...+0.955). The maximum number of stems (924 pcs/m2) and head length (9.2 cm) was demonstrated by the variant with a seeding rate of 4.5 million, height (83 cm) with 5.0 million, productive tillering (3.1) with 5.0–5.5 million, number of grains per head (23.9 pcs.) with 4.0 million, weight of grain per head (1.2 g) with 4.0–4.5 million, 1000-grain weight (47.5 g) with 3.0 million seeds per hectare. It was found that with an increase in the seeding rate, the number of plants increases before harvesting, the number of productive stems, plant height, tillering coefficient and head length increase with r = +0.543...+0.889, but there has been a decrease in indicators such as head weight (r = -0.355) and, especially, 1000-grain weight (r = -0.752). The largest productivity of 7.05 t/ha was obtained with a seeding rate of 4.0 million seeds per hectare, in the second place there was 6.90 t/ha with a rate of 4.5 million seeds per ha. At seeding rates of 4.0–4.5 million germ. seeds per 1 ha, the highest profitability was 67.2–60.2 %.
Increasing interest among agricultural producers in Sarepta spring mustard as a promising, multi-purpose crop requires improving the elements of its cultivation technology to obtain consistently high yields. The current study was carried out in 2021–2022 on the experimental fields of the central experimental base of the FSBSI “FRC “All-Russian Scientific Research Institute of Oil Crops by the name of Pustovoit V. S.”. The purpose of the research was to study and scientifically substantiate individual elements of the technology (sowing time, seed sowing rate and level of mineral nutrition) for Sarepta mustard’s cultivation on seed productivity of the crop grown on leached blackearth (chernozem) of Western Ciscaucasia. There has been established that the highest yield (1.42 t/ha) and oil yield (0.60 t/ha) of the crop is obtained at an early sowing time (the first term (early) is in the 2nd decade of April) with a minimum seed sowing rate (1.3 million units/ha). Sarepta spring mustard’s single feeding of with ammonium nitrate at a dose of N60 in the seedling phase has helped to obtain maximum yield of 2.08 t/ha and 0.87 t/ha of oil. But the largest oil content in seeds (47.1 %) was identified in the variant with the application of nitrogen fertilizer at a dose of N30 fractionally in equal parts, i.e. in the stages of germination (N15) and booting (N15). Foliar treatment of Sarepta spring mustard with helium mixtures (Micro gel at a dose of 1 l/ha and Brassica gel at a dose of 2 l/ha) in the booting stage allowed obtaining the highest yield of 1.85 t/ha, with 47.9 % of oil in seeds and 0.80 t/ha of oil yield.
The purpose of the current study was to establish the effect of sowing rates of spring durum wheat on its productivity. There has been established that reducing seed sowing rates from 7 to 3 million germinated seeds per 1 ha allows reducing spring durum wheat vegetation period by 6–7 days. There has been identified maximum density of productive stems of the two varieties at a seeding rate of 6 and 7 million germinated seeds per 1 ha. The highest productive tillering capacity for two spring durum wheat varieties was obtained at the variant with a norm of 5 million germinated seeds per 1 ha. Thus, according to the experimental variants, the average plant height of the variety ‘Bezenchukskaya Niva’ was 70.6 cm in the control variant and 81.8 cm at a sowing rate of 5 million germinated seeds per 1 ha. The variety ‘Bezenchukskaya Zolotistaya’ has produced the tallest plants of 79.5 cm, with a seeding rate of 4 million germinated seeds per 1 ha. There has been established that the length of a head also increases with a seeding rate decrease; for example, in the variety ‘Bezenchukskaya Niva’, the maximum indicators of the main head were formed at a seeding rate of 5 million germinated seeds per 1 ha, where the maximum length of the main head was 6.7 cm, the number of grains was 27.5 pieces with a weight of 1.47 g. For the variety ‘Bezenchukskaya Zolotistaya’, the maximum head length was 5.6 cm, and the number of grains was 24 pieces at a rate of 4 million germinated seeds per 1 ha. The fullest grain of the variety ‘Bezenchukskaya Niva’ with 53.4 g of ‘1000-grain weight’ was formed at a seeding rate of 5 million germinated seeds per 1 ha; the variety ‘Bezenchukskaya Zolotistaya’ was 50.4g with a norm of 6 million germinated seeds per 1 ha. The maximum productivity of the variety ‘Bezenchukskaya Niva’ was 3.54 t/ha with a seeding rate of 5 million germinated seeds per 1 ha. The variety ‘Bezenchukskaya Zolotistaya’ formed a yield of 3.23 t/ha with a seeding rate of 6 million germinated seeds per 1 ha.
Currently, solving the problem of improving agricultural crops’ productivity is of great relevance. The parameters of photosynthetic activity play an important role in photosynthesis and ensure the efficient functioning of plants under various conditions. Understanding of these processes helps better understand the mechanisms of plant growth and development, as well as develop methods to increase productivity and resistance to stress conditions. The purpose of the current study was to estimate the effect of biofertilizers on the functioning of the photosynthetic apparatus and the variability of the area of leaf blades of agricultural grain crops (durum wheat, spring barley), and to establish the dependence of productivity on the ameliorant used. The objects of study were the spring barley variety ‘Anna’ and the durum wheat variety ‘Orenburgskaya’. The area of the leaf blade was measured by scanning using the APFill Ink&Toner Coverage Meter program. To determine the chlorophyll content, there was used a portable N-tester. There has been reliably established positive correlation between productivity increase and the size of the leaf blade of the experimental plants. The application of the studied biofertilizer has had a positive effect on chlorophyll increase in the barley leaf blades. This explains the possibility of changing the studied parameters by agricultural methods (fertilizer application). There has been found a significant correlation (at p˂0.05) between the height parameters with a number of seeds in a head (r = 0.63) and a leaf blade area (r = 0,69), a number of seeds in a head and productivity (r = 0.86). As a result of the study, there has been established a relationship between some quantitative parameters of the seed material (head length – number of seeds in a head), and a positive correlation was identified between a leaf area and a head length (r = 0.69). There has been obtained a significant dependence of barley productivity on the ameliorant used. At the same time, the productivity increase when using fertilizer compared to the control was 58.9 %.
There have been presented the study results on determining the quality of grain of wintering peas varieties ‘Fokus’ and ‘Zimus’ depending on agricultural methods. The purpose of the current study was to determine protein percentage and yield of basic nutrients in grain of wintering peas, depending on sowing dates and rates. The current study was carried out at the FSBSI “ARC “Donskoy” in 2019–2021. The soil of the experimental plot was represented by ordinary carbonate heavy loamy chernozem. The forecrop was winter wheat. Weather conditions during the years of study were characterized by increased air temperatures in autumn and the return of cold weather in spring, which led to significant damage and thinning of crops. The amount of productive precipitation during the period of grain filling had a positive effect on productivity formation. There has been found that the greatest collection of basic nutrients was obtained in the second ten days of October with a sowing rate of 1.2 and 1.4 million pcs/ha for the varieties ‘Fokus’ and ‘Zimus’, respectively. For the variety ‘Fokus’, the highest crude protein percentage was 25.42 %, with 2.58 t/ha of feed units, 0.557 t/ha of crude protein, 25.31 GJ/ha of metabolic energy, with 189 g of a supply of a feed unit with digestible protein. For the variety ‘Zimus’, the highest crude protein percentage was 25.46 %, with 2.57 t/ha of feed units, 0.555 t/ha of crude protein, 24.62 GJ/ha of metabolic energy, with 190 g of a supply of a feed unit with digestible protein. The studied varieties according to digestible protein percentage were at the level of 205–219 g per a kg of grain.
The Kemerovo region is a region of extreme farming, and therefore requires the development of effective biological methods for improving productivity of cultivated plants adapted to these conditions. The purpose of the current work was to find plant growth-stimulating microorganisms associated with spring barley, as well as to form a microbial consortium on their basis and to conduct field trial. The objects of the study were spring barley seeds selected in the Kemerovo region, growth-stimulating microorganisms isolated from them, and consortia formed on their basis. During the study, there were isolated and tested 9 bacterial isolates for their ability to synthesize growth-stimulating substances (kinetin and indolyl-3-acetic acid). There were selected three strains with the best indicators SHv-2, SHv-5, SHv-6 for further studies. There has been found that the strains are capable of fixing nitrogen (SHv-2 – 800 µg/ml; SHv-5 – 210 µg/ml; SHv-6 – 840 µg/ml). There was also seen an ability to solubilize phosphates (SHv-2 – 2.53; SHv-5 – 1.25; SHv-6 – 3.33). The study of cultural, morphological and biochemical properties made it possible to identify such strains as SHv-2 – Pantoea allii; SHv-5 – Raoultella ornithinolytica; SHv-6 – Pantoea ananatis. Based on these microorganisms, there were made various variants of microbial consortia, which were also valued for their ability to produce growth-promoting substances and fix nitrogen. Consortium MC-7 composed with Pantoea allii, Raoultella ornithinolytica, Pantoea ananatis in a ratio of 1:1:3, respectively, showed maximum activity in terms of the studied parameters. Field trials of this consortium have shown that it successfully improves spring barley productivity. Despite abnormal weather conditions (lack of productive rains), there was an increase in the growth of crops treated by the consortium in comparison with control options. There have been established the most promising options for processing spring barley, options 3 and 4.
ISSN 2079-8733 (Online)