PLANT-GROWING AND PLANT-BREEDING
It has been carried out complex assessment of factors which greatly influence upon harvesting qualitative millet for cereals industry. Quality of millet varieties with different colour of grain with two predecessors (fallow and grain), quality of grain grown in three soil-climatic zones, i.e. southern forestry steppe, north forestry steppe and steppe and quality of millet of various factions have been studied. Grain predecessor of the sowing gave a harvest of large grain (on 2,0-4,1%) and flattened grain (on 1,4%), and fallow predecessor gave a harvest of hulled grain (on 1%) and protein grain (on 1,51%) with less groats harvest (on 1,6%). The varieties with different colours of grain vary in their quality. Groats with the best colour is received from grain of red millet variety with reduced protein content. Variety of white millet possesses higher resistance to hulling (on 2,5-5,8%) of less hulled grain (on 1,0-2,8%). Under processing, factional homogeneity of grain effects on groats yield. The more a portion of medium faction, the lower millet cereal yield is. The best millet grain is formed in the conditions of steppe (Cherlak) in Omsk region on the material of the variety ‘Barnaulskoe 98’. Varieties ‘Saratovsloe Zholtoe’ and ‘Kulundinskoe’ can produce the desired groats raw material in southern forest-steppe (Moskalenki) and steppe (Pavlogradka). The region of north forest-steppe is unfavourable place for production of millet grain with high content of carotenoids. Taking into account a predecessor, region of sowing, variety of millet and factional homogeneity of grain we can significantly differentiate quality of groats under processing.
The use of thermal castration is based on thermal treatment which is fatal for male form of the plant but is acceptable to preserve female fertility. Herewith a small portion of pollen remains viable. In the process of self-pollination with this portion of pollen we receive the seeds which are more viable and adapted to unfavourable environment. Due to this method we managed to cultivate wheat variety ‘Praskoviya’ and a line ‘Zimogor-tk-9’ which significantly exceed variety of winter triticale ‘Zimogor’. The use of the method is expedient not only for constant selection material of later cultivars, but also for new hybrids that will allow improving selection of better economic-valuable forms. Plants of wheat are necessary to be castrated thermally 0,5 or 1,5 days before the alleged anthesis. Water heated up to 480C is a thermal agent. Ears are immersed in hot water for 2 minutes and then are placed under a paper isolator. Necessary equipment for this method of selection includes a thermos flask with hot water, some containers with hot and cold water for regulation of water temperature in a thermos flask after each cycle of castration, a stopwatch, twines, scissors and paper isolators. The use of the method of thermal castration to cultivate valuable self-pollinated forms can be carried out together with hybridization if mother form possesses a strongly marked recessive trait but a father form possesses a dominant one. Thus it becomes possible to distinguish self-pollinated forms and hybrids in the first generation of the hybrid.
In the article the problems of increase of drought and heat tolerance have been considered. The problems connected with application of plant growth regulators to increase productivity, quality of product and crop resistance to abiotic stresses have been considered. The article shows the mechanisms and efficiency of application of growth regulator ‘Zirkon’ (natural mixture of hydroxycinnamic acids and their derivatives extracted from Echinacea purpurea), ‘Epin-Extra’ (analogue of the natural phytohormone epibrassinolide) and silicon-containing chelate micronutrient ‘Siliplant’ for spring and winter wheat and maize under stress. It has been shown that when ‘Epin-Extra’ and ‘Zirkon’ are applied for spring and winter wheat seed preparation and spraying them in the tillering stage, they increase germination, number of productive stems, number of grains in the ear, their mass and productivity in the conditions of moisture shortage and high temperatures. If maize is twice sprayed with these growth regulators in the conditions of extremely high temperatures, the number of cobs per plant, grains per cob and mass of 1000 grains will increase. Fertilizing of ‘Siliplant’ in the tillering stage enhances drought and heat tolerance of grain crops, promotes productive stalk density and improves the main features of the harvest, i.e. ear length, number and mass of grains in the ear.
Imperfect fungus Magnaporthe grisea is a phyto pathogen with a vast influence on most crops including rice. Economic damage of the disease is enormous in all rice cultivation zones of the world. The purpose of the work is to create some cultivars and lines of rice with tree and more genes resistant to pyriculariosis using the method of molecular marking. We introduced and pyramided of Pi-ta, Pi-b, Pi-1, Pi-2, Pi-33 genes resistant to pyriculariosis into domestic cultivars of rice to give them longer resistance to the disease. DNA-markers analysis allowed revealing disease resistant rice samples which possess 5 aimed genes in homozygous condition. The results of phyto pathological testing showed their resistance to a mixture of isolators of M.grisea pathogen, found in Krasnodar Krai. To increase economic efficiency of marker-assisted selection we developed a system of multiplex PCR which can simultaneously identify three Pi-1+Pi-2+Pi-33 genes resistant to M. grisea in a DNA genome of hybrids under one PCR. Its use allows reducing costs of material and time for analyzing of rice samples.
The article reveals basic problems of soybean seed-growing, considers the problems of maintaining of cultivars purity and shows distinctive features of the cultivars. It also sets out indicators of soybean quality and gives their values according to GOST. Because seed quality and genetic homogeneity of a cultivar depend upon both time of reproduction and compliance of cultivation technology, the article reveals technological methods of soybean cultivation, i.e. a place in crop rotation, peculiarities of general and seedbed preparation, a choice of methods and terms of sowing, seeding rates, measures of care for crops and defense from pests and diseases, peculiarities of harvesting and post-harvesting processing and seed storage. The recommendations about optimal soil temperature, seeding rates have been given for the cultivars of different groups of ripeness depending on width of row spacing and depth of seeding. The article deals with the problems of soybean care and the most widespread herbicides for weed control, terms and doses of application. The most harmful pests, permitted substances and doses of application for their control have been listed in the work. We also recommended methods of adjusting soybean harvesters and equipment which can be used for drying and cleaning of seeds. The technology of seed storage is considered in the paper as well. A set of measures to receive soybean seeds with high seeding properties has been recommended.
We suppose its fulfillment can increase quality of primary seed-growing, providing all farms of Rostov region with highly reproductive soybean seeds.Self-pollinated lines of maize have been identified according to the content of alleles of fertility-restorer genes Rf4, Rf5, Rf6 of ‘C’ type of CMS. Genotype of the line SP 286 – rf4 rf4 rf5 rf5 rf6 rf6 possesses restorer genes in recessive state. Genotype of the line SP 357 possesses restorer genes in dominant state (Rf4, Rf5, Rf6). Genotype of the lines SP 275 and SP 331 possesses two restorer genes in dominant state and one gene in recessive state. The study results allow forecasting reaction of the lines on sterile cytoplasm ‘C’ type of CMS. Self-pollinated line SP 286 is going to possess sterile progeny in hybridization with any sterile lines. Fertility will completely be restored in any hybridization with the line SP 357. Fertility restoration in any hybridization with the lines SP 257 and SP 331 will depend on the genotype of the chosen pair. Classification of lines according to the content of fertility-restorer genes will allow finding a suitable program of transferring maize hybrids on a sterile basis and determining position of lines in a hybrid.
AGRICULTURE
It’s common knowledge that heavy metal (HM) can enter the soil together with fertilizers which contaminates agricultural landscapes. The purpose of the research is to study influence of fertilizers on accumulation and migration of Cd, Pb ,Zn, Cu, Co, Mn in soil and plants. The research was conducted during field experiments in chernozem (blacksoil). We studied two levels of plant fertilizing: the first one without fertilizers and the second one with fertilizing of norms of NPK. The fertilizers were distributed in the following way: spring wheat (Triticum vulgare) – N60P60K60; spring barley (Hordeum vulgare) and oats (Avena sativa) – N45P45K20; millet (Panicum miliaceum) – N30P40K40; peas (Pisum sativum) – N10P60K60; buckwheat (Fagopyrum esculentum) – N45P60K60.v We found out that fertilizing raises the amount of Сd, Рb Zn, Сu, Со and Мn on 10-36% in soil and increases mobility of Zn, Си, Со and Мn on 25%. The increase of fertilizing of spring wheat, barley, oats, millet and buckwheat reduces whole volume of HM migration in phyto mass on 5-30%. But it also stimulates Pb migration in the plants of spring wheat, migration of Сd, Zn and Сu into bio mass of oats and barley, and migration of Cd and Mn into peas. The main portion of accumulated elements is stored in a root system of a plant, Zn and Cu are able to transport into inflorescence. The volume of HM accumulation in phyto mass of fertilized plants doesn’t exceed TLV.
PROTECTION AND IMMUNITY
The reaction of barley in callus cultures has been studied using ions Cd2+, Mn2+, Al3+ and PEG as osmotic. Analyzing amount of doses for crop survival, lethal and sublethal metal concentrates were found: manganese (350 and 250 mg/l, respectively), cadmium (30 and 10 mg/l), aluminum (42 and 20 mg/l). Optimal schemes have been suggested to select callus lines tolerant to a complex of stresses. To select a line resistant to complex ion toxicity, it has been recommended to use ions of cadmium (20 mg/l) during a proliferation period and ions of manganese (200 mg/l) during a period of morphogenesis. Ions of aluminum (20-30 mg/l) can be used during both stages of callus tissue development, supporting survival of callus and frequency of plant regeneration. To choose callus with total tolerance to ion toxicity and osmotic stress it’s advisable to apply osmotic (10% of PEG) only during a period of morphogenesis, during a proliferation period it’s possible to use aluminum (20 mg/l) with ions of cadmium (20 mg/l) or ions of manganese (200 mg/l). Using the developed scheme of selection we received 50 plants-regenerators of barley for applying them as starting material in crop selection.
116 cultivars of winter wheat of different ecological-geographical origin were analyzed on resistance to leaf rust (Puccinia triticina Erikss) and powdery mildew (Erysiphe graminis D.C.). These pathogens are the most spread and harmful in Rostov region. The infected plants decrease their productivity on 10-15%, sometimes even on 30%. Cultivars 75/481, Zolotokolosa, Pyvna (Ukraine) and KS 93 U 50 (US) which possess the highest resistance to leaf rust have been selected on the artificially infected allotment. Cultivars IL84-3132-1, KS 93 U 11 (US) and Zrazkova (Ukraine) turned to be the most resistant cultivars (infected on 10-25%). Cultivars KS 96 Wg RC 34 (US), Webster (Canada), Dobirna and Spivanka (Ukraine) turned to be less resistant. The rest cultivars were infected most of all, up to 40% and in some years up to 80-100%. During analyzing the study of winter wheat resistance to leaf rust we found 15 cultivars totally resistant to infection, among which Fokus, Derby, Gaspard (France), Banga (Latvia) and others. Due to long-term study we could select cultivars with a group resistance to leaf rust and powdery mildew. The study results can be used for cultivation of new varieties resistant to fungus diseases.
ECONOMICS
One of the most important priorities of the governmental economic strategy is to form and develop multi structural (mixed) economy as a basis of stable progress of agricultural territories and stable condition of the countryside. Thus, the study of the experience of some regions, enterprises, analysis of the factors and development of practical recommendations is of great importance. The purpose of the study is to monitor of functioning of agricultural enterprises being a part of regional grain productive subcomplex in the in the condition of combination of small and medium agribusiness. In the work the analysis of financial and economic activity of grain farms of Orlov region has been conducted with the help of scientific, economic and statistical methods of study. Features of agricultural production have been revealed taking into consideration sizes and specialization of the enterprises. A complex of enterprises which shows interconnection of productive efficiency with amount of expenditures and subsidies per hectare of grain crops, has been considered. It has been established small and medium agro enterprises develop more efficient but meet preconditions of financial deterioration which can lead to bankruptcy. The conclusion has been made that mixed economy is a principal position of development of domestic grain productive subcomplex nowadays. The results of economic monitoring have been used when making practical recommendations how to increase profitability of agricultural enterprises of Orlov region. The significance of the study is essential under conditions of implementing Governmental Program “Development of Agriculture and Regulation of Agricultural Markets, Raw material and Food during 2013-2020”. The most important tasks of the program are to give support to small farms and to increase profitability in agriculture.
ISSN 2079-8733 (Online)