Identification of short-stem gene Rht-B1 in the winter soft wheat samples
https://doi.org/10.31367/2079-8725-2019-66-6-55-59
Abstract
The current paper has presented the estimation results of the collection material of winter soft wheat according to the presence of alleles of short-stem gene Rht-B1. Identification of the samples with short-stem genes is of great necessity in the development of winter wheat varieties of the steppe ecotype with high adaptability to harvesting. Search, identification and development of a new highly productive and short-stem source material of winter soft wheat is of great relevance nowadays. The purpose of our study was to identify alleles of short-stem gene Rht-B1 in the collection samples of winter soft wheat. The study was carried out in the laboratory of the marker breeding of the FSBSI “Agricultural Research Center “Donskoy” in 2017. There were used the following research methods: a DNA extraction by the CTAB-method, estimation of the quantity and quality of the extracted DNA on a spectrophotometer, fulfillment of polymerase chain reaction and agarose gel electrophoresis, detection of amplicons in ultraviolet by gel photodocumentation system. As a result, due to the use of SSR-markers there were studied 162 short-stem samples of winter soft wheat of various ecological and geographical origin. There were identified 7 winter soft wheat samples with the Rht-B1a allele, such as “Grom”, “Kraplina”, “Stepovichka”, etc. and 13 winter soft wheat samples with the Rht-B1b (Rht1) allele, such as “1765/13”, “1816/15”, “GK Margit”, etc. There were identified 9 winter soft wheat samples with the Rht-B1e (Rht11) allele, such as “GK Margit”, “Minestrel”, “1004/14”, etc. It was recommended to use winter soft wheat samples with the Rht-B1b and Rht-B1e alleles when breeding for short stems.
About the Authors
N. T. Kupreyshvily
Agricultural Research Center “Donskoy”
Russian Federation
technician-researcher of the laboratory of the marker breeding, 347740, Rostov region, Zernograd, Nauchny Gorodok, 3
Russian Federation
technician-researcher of the laboratory of the marker breeding, 347740, Rostov region, Zernograd, Nauchny Gorodok, 3
N. N. Vozhzhova
Agricultural Research Center “Donskoy”
Russian Federation
Candidate of Agricultural Sciences, senior researcher, head of the laboratory of cell breeding, 347740, Rostov region, Zernograd, Nauchny Gorodok, 3
Russian Federation
Candidate of Agricultural Sciences, senior researcher, head of the laboratory of cell breeding, 347740, Rostov region, Zernograd, Nauchny Gorodok, 3
D. M. Marchenko
Agricultural Research Center “Donskoy”
Russian Federation
Candidate of Agricultural Sciences, leading researcher, head of the department for winter wheat breeding and seed production, 347740, Rostov region, Zernograd, Nauchny Gorodok, 3
Russian Federation
Candidate of Agricultural Sciences, leading researcher, head of the department for winter wheat breeding and seed production, 347740, Rostov region, Zernograd, Nauchny Gorodok, 3
E. V. Ionova
Agricultural Research Center “Donskoy”
Russian Federation
Doctor of Agricultural Sciences, head of the center of fundamental researches, deputy director on science, 347740, Rostov region, Zernograd, Nauchny Gorodok, 3
Russian Federation
Doctor of Agricultural Sciences, head of the center of fundamental researches, deputy director on science, 347740, Rostov region, Zernograd, Nauchny Gorodok, 3
References
1. D'yachuk T. I., Kibkalo I. A., Pominov A. V. i dr. Perspektivnye linii v selekcii tritikale dlya uslovij Povolzh'ya [Promising lines in the triticale breeding in the Volga region] // Zernovoe hozyajstvo Rossii. 2018. № 5(59). S. 39–43. DOI: 2079-8725-2018-59-5-39-43.
2. Medvedev A. M., Poma N. G., Osipov V. V. i dr. Poisk istochnikov korotkostebel'nosti v celyah sozdaniya ustojchivyh k poleganiyu sortov ozimoj tritikale dlya Central'nogo Nechernozem'ya [Search for sources of short-stem in order to create the lodging resistant winter triticale varieties for the Central Non-Chernozem Region] // Zernovoe hozyajstvo Rossii. 2017. № 3(51). S. 43–46.
3. Merezhko A. F. Problema donorov v selekcii rastenij [The problem of donors in plant breeding]. SPb.: VIR, 1994. 128 s.
4. Samofalova N. E. Mechta i yav' akademika I. G. Kalinenko v sozdanii ozimoj tverdoj pshenicy [The dream and reality of the academician I. G. Kalinenko in the development of winter durum wheat] // Zernovoe hozyajstvo Rossii. 2016. № 1(43). S. 1–9.
5. Chebotar' S. V. Allel'naya harakteristika genov korotkostebel'nosti v geneticheskom pule sortov ozimoj myagkoj pshenicy Ukrainy [Allelic characteristics of short-stem genes in the genetic pool of varieties of winter soft wheat of Ukraine] // Genetichni resursi roslin. 2008. T. 6. S. 96–103.
6. Divashuk M., Bespalova L., Vasilyev A., Fesenko I., Puzyrnaya O., Karlov G. Reduced height genes and their importance in winter wheat cultivars grown in southern Russia // Euphytica. 2013. Vol. 190, no. 1. Pp. 137–144. https://doi.org/10.1007/s10681-012-0789-7.
7. Ellis M., Spielmeyer W., Gale K., Rebetzke G., Richards R. “Perfect” markers for the Rht-B1b and Rht-D1b dwarfing genes in wheat // Theoretical and Applied Genetics. 2002. Vol. 105, no. 6-7. Pp. 1038–1042. https://doi.org/10.1007/s00122-002-1048-4.
8. FAO Global Statistical Yearbook // Book FAO Global Statistical Yearbook / Editor, 2019. Available at: http://www.fao.org/faostat/en/#data/QC.
9. Guedira M., Brown-Guedira G., Van Sanford D., Sneller C., Souza E., Marshall D. Distribution of Rht Genes in Modern and Historic Winter Wheat Cultivars from the Eastern and Central USA // Crop Science. 2010. Vol. 50, no. 5. Pp. 1811–1822. https://doi.org/10.2135/cropsci2009.10.06.0626.
10. Knopf C., Becker H., Ebmeyer E., Korzun V. Occurrence of Three Dwarfing Rht Genes in German Winter Wheat Varieties // Cereal Research Communications. 2008. Vol. 36, no. 4. Pp. 553–560. https://doi.org/10.1556/CRC.36.2008.4.4.
11. Li A., Yang W., Guo X., Liu D., Sun J., Zhang A. Isolation of a gibberellin-insensitive dwarfing gene, Rht-B1e, and development of an allele-specific PCR marker // Molecular Breeding. 2012. Vol. 30, no. 3. Pp. 1443–1451. https://doi.org/10.1007/S11032-012-9730-y.
12. Murray M. G., Thompson W. F. Rapid isolation of high molecular-weight plant DNA // Nucleic Acids Research. 1980. Vol. 8, no. 19. Pp. 4321–4325. https://doi.org/10.1093/nar/8.19.4321.
Review
For citations:
Kupreyshvily N.T., Vozhzhova N.N., Marchenko D.M., Ionova E.V. Identification of short-stem gene Rht-B1 in the winter soft wheat samples. Grain Economy of Russia. 2019;(6):55-59. (In Russ.) https://doi.org/10.31367/2079-8725-2019-66-6-55-59
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