Using retrotransposons to assess the genetic diversity of a tomato collection
https://doi.org/10.31367/2079-8725-2026-102-1-50-57
Abstract
Modern agriculture places high demands on new varieties and hybrids when cultivating any crop. Therefore, to correctly select parental forms, breeders must have precise characteristics and an understanding of the future variety’s morphotype. Modern methods of molecular marking using highly polymorphic marker systems significantly simplifies the estimation of the diversity of collection forms, the sources of donors of economically valuable traits, by marking target genes. In order to evaluate 70 collection tomato samples from VIR with different ecological and geographical origins, to identify potential donors of resistance to bio- and abiotic stressors, there has been conducted their genetic analysis based on PCR using iPBS markers. There have been tested 15 iPBS markers in the amplification reaction, only ten of which have demonstrated high levels of polymorphism in the analyzed samples. There have been identified 1,923 alleles ranging from 100 to 3,000 bp. The marker 2273 (271 bands) has demonstrated the highest polymorphism with 90.75%. Thus, testing of iPBS markers to estimate the genetic diversity of tomato has shown the possibility of their use, which is confirmed by statistical analysis of the allele frequency, and they can be recommended as an effective method in studying the biodiversity of both collection and breeding forms of tomato.
About the Authors
A. L. Nazarov
FSBEI HE “Kuban State Agrarian University named after I.T. Trubilin”
Russian Federation
Russian Federation
postgraduate
350044, Krasnodar, Kalinin Str., 13
E. V. Dubina
FSBEI HE “Kuban State Agrarian University named after I.T. Trubilin”; FSBSI “Federal Scientific Rice Centre”
Russian Federation
Russian Federation
Doctor of Biological Sciences, professor of RAS, professor of the department of genetics, breeding and seed production
350044, Krasnodar, Kalinin Str., 13
350921, Krasnodar Region, Krasnodar, Belozerny, 3
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Review
For citations:
Nazarov A.L., Dubina E.V. Using retrotransposons to assess the genetic diversity of a tomato collection. Grain Economy of Russia. 2026;18(1):50-57. (In Russ.) https://doi.org/10.31367/2079-8725-2026-102-1-50-57
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