ACTIVITY OF POLYPHENOL OXIDASE IN SORGHUM GRAIN (SORGHUM BICOLOR L.)
Abstract
Grain of grain sorghum has different ways of use. In many countries of the world grain of sorghum is applied for cooking different food products. Thus grain of sorghum is imposed certain requirements in dependence of the purpose of its use. The article gives the results of study of activity of polyphenol oxidase in grain of 49 varieties and selective lines of grain sorghum of various origins. In the trials we used grain with different 1000-grain weight and color. 1000-grain weight varied from 10,9 to 48,5 g. Grain color was presented with a total color spectrum. It has been determined that the activity of polyphenol oxidase (PPO) in grain of studied sorghum samples varied from 2,83 to 21.01 u/g. The majority of sorghum samples of I.G. Kalinenko ARRIGC possess low activity of PPO. It has been shown that the activity of PPO negatively correlates with grain weight (r=-0,76) and positively correlates with grain color (r=0,49). The activity of PPO can result in darkening of the product, reducing its quality. For cooking of the most food product from grain of sorghum it’s necessary to use grain of light colored varieties of sorghum with the low activity of PPO.
About the Authors
A. A. Yatsyna
FSBSI All-Russian Research Institute of Grain Crops named after I.G. Kalinenko
Russian Federation
Russian Federation
Candidate of Biological Sciences.
347740, Zernograd, Nauchny Gorodok, 3
V. V. Kovtunov
FSBSI All-Russian Research Institute of Grain Crops named after I.G. Kalinenko
Russian Federation
Russian Federation
Candidate of Agricultural Sciences.
347740, Zernograd, Nauchny Gorodok, 3
References
1. Taylor, J.R.N. Novel food and non-food uses for sorghum and millets/ J.R.N.Taylor, T.J.Schober, S.R.Bean// J.Cereal Sci. – 2006. – V. 44. – P. 252-271.
2. Lyumugabe, F. Characteristics of African traditional beers brewed with sorghum malt: a review/ F. Lyumugabe, J. Gros, Nzungize, E. Bajyana, P. Thonart// Biotechnol. Agron. Soc. Environ. -2012. – V. 16. – P. 509-530.
3. Hikeezi, D.M. Polyphenol oxidase activity in white tan-plant-type sorghum: an important determinant of the relatively dark color of their porridges/ D.M. Hikeezi, K.G. Duodu, M. Chisi, L.W. Rooney, J.R.N. Taylor// Intern. J. Food Sci. Technology. 2013. V. 48. – P. 941-946.
4. Dykes L., Rooney L.W. Sorghum and millet phenols and antioxidants// J. Cereal Sci. 2006. – V. 44. – P. 236-251.
5. Awika, J.M., Rooney L.W. Sorghum phytochemicals and their potential impact on human health// Phytochemistry. 2004. – V. 65. – P. 1199-1221.
6. Shepel, N.A. Sorghum / N.A. Shepel. – Volgograd: Committee on printing, 1994. – 448p.
7. Khusnetdinova T.G. Creating of initial material for sorghum breeding for food. Synopsis of Cand. of Agric. Sc.. Saratov, 1997.
8. Mayer, A.M. Polyphenol oxidases in plants and fungi: Going places? A review // Phytochemistry. -2006. – V. 67. – P. 2318-2331.
9. Queiroz, C. Polyphenol oxidase: characteristics and mechanism of browning control / C.Queiroz, M.L.M. Lopes, E. Fialho, V.L. Valente-Mesquita // Food Rew. Intern. – 2008. – V. 24. – P. 361-375.
10. Feillet, P. Pasta brownness: an assessment / P. Feillet, J. Autran, C. Icard-Verniere // J. Cereal Sci. 2000. – V. 32. – P. 215-233.
11. Dicko, M.H. Phenolic compounds and related enzymes as determinants of sorghum for food use / M.H. Dicko, H. Gruppen, A.S. Traore, A.G.J. Voragen, W.J.H.van Berkel // Biotechnology and Molecular Biology Review. 2006. – V. 1. – P. 21-38.
12. Anderson, J.V., Morris, C.F. An improved whole-seed assay for screening wheat germplasm for polyphenol oxidase activity / J.V. Anderson, C.F. Morris // Crop Sci. – 2001. – V. 41. – P. 1697-1705.
13. Yakushevsky, E.S. The broad unified comecon and international comecon list of descriptors for the species ‘Sorghum Moench’ / E.S. Yakushevsky, S.G. Varadinov, V.A. Korneychuk, L. Banyai. – L.: VIR, 1982. – 34p.
14. Dicko, M.H. Comparison of content in phenolic compounds, polyphenol oxidase, and peroxidase in grains of fifty sorghum varieties from Burkina Faso / M.H. Dicko, R. Hilhorst, H. Gruppen, A.S. Traore, C. Laane, W.J.H. van Berkel, A.G.J. Voragen // J.Agric.Food Chem. – 2002. – V. 50. – P.3780-3788.
15. Kang, C.S. Effect of polyphenol oxidase activity on discoloration of noodle dough sheet prepared from Korean wheat / C.S. Kang, Y.K. Cheong, S.L. Kim, D.K. Kim, J.G. Kim, C.S. Park // Korean J. Crop Sci. – 2008. – V. 53. – P. 187-195.
16. Park, Y.H. Genotype and environment effects on gliadin content and poly phenol oxidase activity in wheat/ Y.H.Park, B.H. Hong, M.W. Park, J.H. Nam, Y.W. Seo // Korean J. Crop Sci. – 2000. – V. 45. – P. 38-43.
17. Sayaslan, A. Some quality characteristics of durum wheat (Triticum durum) landraces / A.Sayaslan, M. Koyuncu, A. Yildirim, T. Eserkaya Gulec, O.Ates Sonmezoglu, N. Kandemir // Turk.J.Agric.For. – 2012. – V. 36. – P. 749-756.
Review
For citations:
Yatsyna A.A., Kovtunov V.V. ACTIVITY OF POLYPHENOL OXIDASE IN SORGHUM GRAIN (SORGHUM BICOLOR L.). Grain Economy of Russia. 2015;(6):61-71. (In Russ.)
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