Estimation of stability of DNA-containing vegetable samples stored under the accelerated DNA degradation
https://doi.org/10.31367/2079-8725-2020-67-1-69-71
Abstract
There has been carried out an estimation of stability of DNA-containing plant samples stored under the conditions that model accelerated DNA degradation. The research material were the lines H. annuus (“VIR 114”, “VIR 116”) and wild-growing forms of sunflower H. decapetalus, H. petiolaris, H. rigidus taken from the collection of the N. I. Vavilov All-Russian Institute of Plant Genetic Resources. On average, the stability level of long DNA fragments for the nuclear, mitochondrial, and chloroplast genomes was 76.9%, 87.5%, and 98.3% respectively compared with the control. When assessing the stability of short DNA fragments, it was shown that on average, the number of nuclear, mitochondrial, and chloroplast DNA was 89.5%, 96.0%, and 97.7% respectively compared with the control.
About the Authors
V. A. Khachumov
Academy of biology and biotechnology, the Southern Federal University
Russian Federation
Russian Federation
Post graduate.
344090 Rostov-on-Don, Stachki Av., 194/1.
I. V. Kornienko
Academy of biology and biotechnology, the Southern Federal University
Russian Federation
Russian Federation
Doctor of Biological Sciences.
344090 Rostov-on-Don, Stachki Av., 194/1.
A. V. Usatov
Academy of biology and biotechnology, the Southern Federal University
Russian Federation
Russian Federation
Doctor of Biological Sciences, professor.
344090 Rostov-on-Don, Stachki Av., 194/1.
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Review
For citations:
Khachumov V.A., Kornienko I.V., Usatov A.V. Estimation of stability of DNA-containing vegetable samples stored under the accelerated DNA degradation. Grain Economy of Russia. 2020;(1):69-71. (In Russ.) https://doi.org/10.31367/2079-8725-2020-67-1-69-71
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