THE DEVELOPMENT STAGES OF THE RICE VARIETY ‘PARTNER’ WITH A GENE OF RESISTANCE TO RICE BLAST DISEASE Pi-B

Blast disease is a widely spread and harmful disease of rice. The most reliable method to fight with it is a development of resistant varieties. The article presents the development stages of the new rice variety 'Partner' with higher resistance to blast disease. It has been shown that on the basis of DNA-markers we developed breeding material with a gene of resistance to blast disease Pi-b. The donor of the gene Pi-b was the later maturing Japanese variety 'BL-1'. The recipient was the variety 'Yantar' cultivated in theKrasnodararea. The obtained material allowed identifying and studying 20 lines in the breeding seed plot. The estimation of the material in the breeding seed plot resulted in the identification of five best lines and their introduction into the control seed plot. According to the complex of economic traits two variety samples 'KP 117-09' and 'KP 126-09' were identified. The variety sample 'KP 117-09' showed 24.9 % of blast disease development during artificial infection, 'KP 126-09' had 27.7%. The rest varieties including the standard variety 'Rapan' had a larger percent of infection (42.7%). In 2011-2013 the identified samples were subjected to a competitive testing. According to the results the variety sample 'KP 117-09' (named as 'Partner') was found the best and was sent to the State Variety Testing. On average the variety 'Partner' showed better productivity that 'Rapan' (8.54 and 7.93 t/ha respectively); it surpassed the standard variety in the trait '1000-kernel weight' (32.3 gvs 28.0g) and in resistance to blast disease (22.6% vs 48.7%). The other traits of the comparing varieties had the close values. In 2013 the new variety 'Partner' was sent to the State Variety Testing. Due to two years of study it was introduced into the List (Register) of Breeding Achievements and since 2016 it was approved for use in the Krasnodar Area. 

1. Zelensky, G.L. The fight with rice blast disease by the developing of stable varieties/ G.L. Zelensky. – Krasnodar: KubSAU, 2013. – 92 p.

2. Suprun, I.I. Breeding and molecular-genetic methods in the developing of the rice lines resistant to blast disease / I.I. Suprun, V.N. Shilovsky, V.Ya. Ruban // Vestnik RAA, 2012. – № 1. – PP. 60-62.

3. Suprun, I.I. The creation of Pi-b, an intragenic DNA-marker of resistance to blast disease and its use in practical breeding / I.I. Suprun, E.T. Ilnitskaya, Zh.M. Mukhina // Agricultural biology, 2007. – № 5. – PP. 63-66.

4. Murray, M.G. Rapid isolation of high molecular weight plant DNA / M.G. Murray, W.F. Thompson // Nicleic Acids Research, 1980. – V 80. – P. 4321-4325.

5. Shibata, D.K. Polymerase chain reaction and molecular-genetic analysis of biopsy // Molecular of clinic diagnostics. – М.: Mir, 1999. – PP. 395-427.

6. Smetanin, A.P. Methods of experiments in breeding, seed-growing and control of rice quality / A.P. Smetanin, V.A. Dzyuba, A.I. Aprod. – Krasnodar, 1972. –186 p.

7. Sazonov, V.I. Agricultural experience in plant-growing and its methodology / V.I. Sazonov. – Selkhozizdat, 1962. – PP. 69-81.

8. Kovalev, V.S. The improvement of the methodology and technology of starting of a competitive variety testing of rice / V.S. Kovalev, N.V. Ostapenko // Abstracts of the reports of the confer. for young researchers and specialists. – Krasnodar, 1987. – PP. 10-12.

9. Dospekhov, B.A. Methodology of a field trial / B.A. Dospekhov. – Moscow: Kolos, 1979. – 416 p.

10. Sheydzhen, A.Kh. Agrochemistry. Part 2. Methodology of agrochemical researches / A.Kh. Sheydzhen, T.N. Bondareva. – Krasnodar: KubSAU, 2015. – 702 p.