Estimation of efficiency of in vitro androgenesis in the production of doubled rice haploids
https://doi.org/10.31367/2079-8725-2025-101-6-47-55
Abstract
Rice is an important food crop, used to feed a large portion of the world’s population. The use of in vitro anther culture makes possible to develop homozygous offspring and genetically unique material within 1–2 years. The purpose of the current study was to estimate the efficiency of anther cultures and the production of doubled rice haploids. The number of calli formed within the hybrids ranged from 0 to 819. The callus formation rate varied from 0 to 115 %. There has been found that eleven rice hybrids (61 %) formed callus. During callus cultivation on regeneration medium, there have been developed 137 green and 108 albino plants. There was a low regeneration rate of 1.6% among the regenerated plants. The largest number of calli and large productivity of regenerated plants were found in such three hybrids as ‘Klassik x Virasan’ with 568 and 23 pcs., ‘Rapan 2 x Argamak’ with 358 and 32 pcs. and ‘IR86385-248-2-1-B x Kontakt’ with 819 and 152 pcs., respectively. The largest number of regenerated plants after adaptation in soil was obtained from the hybrid sample ‘IR86385-248-2-1-B x Kontakt’ (41 pcs.), including 4 doubled haploids, and the remaining 37 haploids. Doubled haploid regenerated plants have formed panicles with high fertility (84.5%). The lines ‘IR86385-248-2-1-B x Kontakt’ and ‘Rapan 2 x Argamak’ were included in the further breeding process.
About the Authors
N. G. Chertkova
FSBSI Agricultural Research Center “Donskoy”
Russian Federation
Russian Federation
N.G. Chertkova, junior researcher of the laboratory for cell breeding
347740, Rostov region, Zernograd, Nauchny Gorodok, 3
N. V. Kalinina
FSBSI Agricultural Research Center “Donskoy”
Russian Federation
Russian Federation
N.V. Kalinina, researcher of the laboratory for cell breeding
347740, Rostov region, Zernograd, Nauchny Gorodok, 3
V. Yu. Dontsova
FSBSI Agricultural Research Center “Donskoy”
Russian Federation
Russian Federation
V.Yu. Dontsova, junior researcher of the laboratory for cell breeding
347740, Rostov region, Zernograd, Nauchny Gorodok, 3
P. I. Kostylev
FSBSI Agricultural Research Center “Donskoy”
Russian Federation
Russian Federation
P.I. Kostylev, Doctor of Agricultural Sciences, main researcher of the laboratory for rice breeding and seed production
347740, Rostov region, Zernograd, Nauchny Gorodok, 3
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Review
For citations:
Chertkova N.G., Kalinina N.V., Dontsova V.Yu., Kostylev P.I. Estimation of efficiency of in vitro androgenesis in the production of doubled rice haploids. Grain Economy of Russia. 2025;17(6):47-55. (In Russ.) https://doi.org/10.31367/2079-8725-2025-101-6-47-55
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