Pcr-analysis of new rice varieties for the presence of blast resistance genes
https://doi.org/10.31367/2079-8725-2025-101-6-38-46
Abstract
Rice is a basic food crop worldwide, but rice blast can cause significant yield losses, costing the global economy over $70 billion. Therefore, the development of new blast-resistant rice varieties is crucial. Due to the accumulated progress in studying the genetic mechanism of rice resistance to blast, there have been found many resistance genes (or quantitative trait loci (QTL)), the pyramiding of a number of which in one genotype can improve resistance and accelerate the development of new resistant rice varieties. The purpose of the current study was to identify and select rice lines with high productivity potential and carrying five blast resistance genes Pi-1, Pi-2, Pi-33, Pi-b, and Pi-ta. These resistance genes were identified using molecular genetic methods, including DNA extraction, PCR with specific molecular markers linked to the resistance genes, electrophoresis, and electropherogram analysis. During the analysis, there have been identified rice samples of the control nursery and competitive variety testing that carried five resistance genes Pi-1, Pi-2, Pi-33, Pi-b, and Pi-ta in their genotypes. These were middle-maturing samples, with 120 days of vegetation from sowing to full maturity. The plants were 90–100 cm tall, with upright leaves and panicles. The panicles were compact, 14–16 cm, and had 120–180 medium-sized, oval-shaped spikelets. The mean value of 1000-grain weight was 30–32 g. The varieties were resistant to blast, lodging, and shattering. The productivity in the competitive variety testing (2023-2024) averaged 7.64 t/ha, exceeding that of the standard ‘Yuzhanin’ by 1.50 t/ha.
About the Authors
P. I. Kostylev
FSBSI Agricultural Research Center “Donskoy”
Russian Federation
Russian Federation
P.I. Kostylev, Doctor of Agricultural Sciences, professor, main researcher of the laboratory for rice breeding and seed production
347740, Rostov region, Zernograd, Nauchny Gorodok, 3
N. N. Vozhzhova
FSBSI Agricultural Research Center “Donskoy”
Russian Federation
Russian Federation
N.N. Vozhzhova, Candidate of Agricultural Sciences, senior researcher of the laboratory for cell breeding
347740, Rostov region, Zernograd, Nauchny Gorodok, 3
A. V. Aksenov
FSBSI Agricultural Research Center “Donskoy”
Russian Federation
Russian Federation
A.V. Aksenov, junior researcher of the laboratory for rice breeding and seed production
347740, Rostov region, Zernograd, Nauchny Gorodok, 3
E. V. Krasnova
FSBSI Agricultural Research Center “Donskoy”
Russian Federation
Russian Federation
E.V. Krasnova, Candidate of Agricultural Sciences, leading researcher of the laboratory for rice breeding and seed production
347740, Rostov region, Zernograd, Nauchny Gorodok, 3
References
1. Ilyushko M.V., Romashova M.V., Guchenko S.S. Molekulyarnoe markirovanie genov ustoichivosti k pirikulyariozu v sortakh risa dal'nevostochnoi selektsii [Molecular marking of blast resistance genes in rice varieties of the Far Eastern breeding] // Agrarnaya Rossiya. 2020. № 10. P. 30–33. DOI: 10.30906/1999-5636-2020-10-30-33
2. Dubina E.V., Ruban M.G., Lesnyak S.A., Kutishcheva A.A. Sozdanie s pomoshch'yu DNK-markerov novykh genotipov risa, ustoichivykh k pirikulyariozu [Development of new rice genotypes resistant to blast using DNA markers] // Geneticheskii potentsial sel'skokhozyaistvennykh rastenii i ego realizatsiya v selektsii, semenovodstve i razmnozhenii. Vserossiyskaya nauchno-prakticheskaya konferenciya Kubanskogo otd. VOGiS. Krasnodar, 2024. P. 63–66.
3. Nartymov D.V., Dubina E.V., Ruban M.G., Aniskina Yu.V., Garkusha S.V., Shilov I.A., Velishaeva N.S., Kolobova O.S., Istomin N.K. Izuchenie geneticheskogo raznoobraziya vozbuditelya pirikulyarioza risa Pyricularia oryzae Sav. po kompleksu molekulyarnykh i morfologicheskikh priznakov [Study of the genetic diversity of the rice blast pathogen Pyricularia oryzae Cav. according to a complex of molecular and morphological characteristics] // Genetika. 2023. T. 59, № 6. S. 640–647. DOI: 10.31857/S0016675823060127
4. Guo L., Zhao H., Wang J., Liu H., Zheng H., Sun J., Yang L., Sha H., Zou D. Dissection of QTL alleles for blast resistance based on linkage and linkage disequilibrium mapping in japonica rice seedlings // Australasian Plant Pathol. 2016. Vol. 45. P. 209–218. DOI: 10.1007/s13313-016-0405-8
5. Manoj C.S., Vipul P.P., Chirag P.C., Patel V.B., Denish S., Himani P.V., Kaushal M., Kedar N., Patel R.K. Molecular characterization and phenotypic selection for blast resistance and yield enhancement in rice (Oryza sativa L.) // Plant Sci. Today. 2025. Vol. 12 (3). P. 1–11. DOI: 10.14719/pst.7726
6. Scheuermann K.K., Raimondi J.V., Marschalek R., de Andrade1 A., Wickert E. Magnaporthe oryzae genetic diversity and its outcomes on the search for durable resistance. // The Molecular Basis of Plant Genetic Diversity, Prof. Mahmut Caliskan (Ed.), ISBN: 978-953-51-0157-4, InTech, Available from: http://www.intechopen.com/books/the-molecular-basis-of-plant-genetic-diversity/magnaporthe-oryzae-geneticdiversity-and-its-outcomes-on-the-search-for-durable-resistance. 2012. P. 331–356. DOI: 10.5772/33479
7. Wang Z.X., Yano M., Yamanouchi U., Iwamoto M., Monna L., Hayasaka H., Katayose Y., Sasaki T. The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine rich repeat class of plant disease resistance genes // Plant Journal. 1999. Vol. 19(1). P. 55–64. DOI: 10.1046/j.1365-313x.1999.00498.
8. Wu Y., Xiao N., Chen Y., et al. Comprehensive evaluation of resistance effects of pyramiding lines with different broad-spectrum resistance genes against Magnaporthe oryzae in rice (Oryza sativa L.) // Rice. 2019. V. 12. article number 11. DOI: 10.1186/s12284-019-0264-3
Review
For citations:
Kostylev P.I., Vozhzhova N.N., Aksenov A.V., Krasnova E.V. Pcr-analysis of new rice varieties for the presence of blast resistance genes. Grain Economy of Russia. 2025;17(6):38-46. (In Russ.) https://doi.org/10.31367/2079-8725-2025-101-6-38-46
Views:
12
JATS XML
Email this article 