THE USE OF DNA - TECHNOLOGIES FOR CREATION AND IMPRVEMENT OF INITIAL STOCK FOR BREEDING OF COLD TOLERANT RICE VARIETIES

For Russia countries with a temperate climate, with direct sowing of rice increase of cold tolerant varieties in seed germination and shoot formation are important. This trait allows to begin rice sowing earlier using for plant growing the favorable period by temperature to obtain optimum shoots by density that gives the chance to realize potential efficiency of varieties. A research objective is to create and estimate the initial stock resistant to low positive temperatures for use it for breeding of new cold tolerant rice varieties.Display of molecular markers - is neutral in relation to a phenotype and they can be found out at any stage of development of plants in ontogenesis. Methods of DNA-genotipirovanija and PCR analysis by means of molecular markers allow accelerating carrying over of the necessary genes in the course of breeding and to provide creation of new varieties with a complex of the set properties Therefore the role of biotechnological methods is important at creation new cold tolerant varieties.According to results of PCR analysis by use of SSR - marker lines of rice with dominant alleles of cold resistance genes were screened. Double haploids were obtained by anther culture method in vitro and resistant forms were found. Plants with economically valuable traits were screened. All-round evaluation of obtained material gave the possibility to find initial stock for use breeding process in future for release of new cold resistant rice varieties. 

1. Vorobyov, N.V. Physiology of rice seed germination of of / Vorobyov Nikolai Vasilyevich: abstract of thesis …D.Sc. - М, Т.A.A., 1986. – 31 p.

2. Vorobyov, N.V. Physiological bases of rice seed germination and methods of germination power increase // N.V. Vorobyov. – Krasnodar, 2003. – 116 p.

3. Skazhennik, M.A. Creation of cold tolerant initial stock for rice breeding in the frame work of Consortium of temperate climate countries / M.A Skazhennik, N.V. Vorobyov, V.A. Dzuba [et al.] // Grain crop farms of Russia. – 2013. – № 3(27). – P. 11-15.

4. Butenko, R,G. Technology in vitro in agriculture / R.G. Butenko // Agriculture biology. – 1983. – № 5. – P. 3-7.

5. Malysheva, N.N. Obtaining, evaluation and screening of rice double haploid lines with good economical traits / N.N. Malysheva, E.G, Savenko, V.A. Glazyrina, [et al.] // Rice growing. – 2012. – № 2(21). – P. 14-18.

6. Glazko, V.I. Vvedenie in genetics, biocomputer science, DNA-technology, DNA-ecology, proteomika, metabolica / V.I. Glazko, T.V. Glazko. – Krasnodar, 2003. – 640 p.

7. Dzuba, V.A. The use of innovative approaches in rice breeding / V.A. Dzuba, L.V. Yesaulova, I.N. Chukhir // Scientifically-innovative bases of development рисоводства in Kazakhstan and the abroad countries: materials of the International scientifically-practical conference. – Kyzylorda. – 2012. – C. 75-78.

8. Los, G.D. Perspective method of rice hybridization /G.D. Los,.// Agriculture biology. – 1987. – № 12. – P.107 -109.

9. Murray, M.G. Rapid isolation of high molecular weight plant DNA / M.G. Murray, W.F. Thompson // Nucleic Acids Research. – 1980. – № 10. – P. 4321-4325.

10. Smetanin, A.P. Methods of experiments on breeding, seed-production and rice seed quality control / A.P. Smetanin, V.A. Dzuba, A.I. Aprod // Krasnodar, 1972. – 156 p.

11. Dzuba, V.A. Multifactor experiments and methods of biometric analysis and experimental data / V.A. Dzuba. – Krasnodar, 2007. – 76 p.

12. Skazhennik, M.A. Creating of tolerant initial material of rice for selection of the cultivars in consortium of countries with moderate climate / M.A Skazhennik, N.V. Vorobyov, V.A. Dzuba [et al.] // Grain crop farms of Russia. – 2014. – № 5(35). – P. 11-17.