The effect of biofertilizer on water regime, intensity of photosynthesis and productivity of grain crops in the Steppe Pre-Urals

Grain crops are of great food value, productivity of which largely depends on the water regime and assimilation activity of plants. The purpose of the current study was to determine the effect of biofertilizer on the parameters of the water regime, pigment, and gas composition of grain crops. The study was conducted at the stationary research site of the Botanical Garden of Orenburg State University in 2021–2023. The objects of the research were the spring barley variety Anna, the spring common wheat variety Orenburgskaya Yubileinaya and the spring durum wheat variety Orenburgskaya. The experiment was carried out using biofertilizer containing fermented food residues and crushed plant waste with B. Subtilis bacteria immobilized on their surface. The water regime parameters were studied according to the method of N.N. Kozhushko. Photometric diagnostics was carried out using a portable N-tester device (model PRAK 431155.022, manufactured by Agrotester LLC, Russia). The CO₂ content was measured with a portable gas analyzer CI-340 (“CI-340 Hand-held Photosynthesis System”, manufactured by CID Bio-Science, USA). Statistical data processing was performed using the program Statistica 10.0. Based on the study results of the studies, there was found similar dynamics of the decrease in total water content and transpiration intensity for the studied plants at the end of the vegetation period. There has been established a water deficit increase of grain crops in milk stage and its reliable decrease in the biomass of spring common wheat and barley when using biofertilizers in comparison with the control variants. The maximum value of the pair correlation coefficient was established between the parameters of carbon dioxide content and transpiration intensity in common wheat and barley. As for nitrogen in leaves, there has been found a relative constancy of the indices in the samples when using biofertilizer. In contrast, the control samples have shown variability of this trait. There has been identified a positive effect of biofertilizers on the parameters of the water regime of the grain crops, as well as the intensification of photosynthetic activity when using this ameliorant, which improved productivity of the studied grain crops by 147.1 % for barley, and by 105.7 % for common wheat.

1. Kozhushko N. N. Otsenka zasukhoustoichivosti polevykh kul'tur [Estimation of drought resistance of field crops. Diagnostics of plant resistance to stress effects (methodological guide)]. Diagnostika ustoichivosti rastenii k stressovym vozdeistviyam (metodicheskoe rukovodstvo) / pod red. G. V. Udovenko. L., 1988. VIR. 49 s.

2. Patent № 2824464 C1 Rossiiskaya Federatsiya, MPK C05F 11/08, C05F 9/04. Bioorganicheskoe udobrenie [Patent No. 2824464 C1 Russian Federation, IPC C05F 11/08, C05F 9/04. Bioorganic fertilizer]: № 2023133113: zayavl. 14.12.2023: opubl. 08.08.2024 / L. V. Galaktionova, N. A. Terekhova, D. G. Fedorova i dr.; zayavitel' Federal'noe gosudarstvennoe byudzhetnoe obrazovatel'noe uchrezhdenie vysshego obrazovaniya «Orenburgskii gosudarstvennyi universitet».

3. Deng X., Liu Y., Xu X. X., Liu D. M., Zhu G. R., Yan X., Wang Z. M., Yan Y. M. Deng X. et al. Comparative proteome analysis of wheat flag leaves and developing grains under water deficit // Frontiers in Plant Science. 2018. Vol. 9, Article number: 425. DOI: 10.3389/fpls.2018.00425

4. Duan W., Wang S., Zhang H., Xie B., Zhang L. Plant growth and nitrate absorption and assimilation of two sweet potato cultivars with different N tolerances in response to nitrate supply // Scientific Reports. 2024. Vol. 14(1), Article number: 21286. DOI: 10.1038/s41598-024-72422-y

5. Janeeshma E., Johnson R., Amritha M. S., Noble L., Aswathi K. P. R., Telesiński A., Kalaji H. M., Auriga A., Puthur J. T. Modulations in Chlorophyll a Fluorescence Based on Intensity and Spectral Variations of Light // Journal of Experimental Botany. 2022. Vol. 23(10), Article number: 5599. DOI: 10.3390/ijms23105599

6. Kumar S., Sindhu S. S. Drought stress mitigation through bioengineering of microbes and crop varieties for sustainable agriculture and food security // Current Research in Microbial Sciences. 2024. Vol. 10(7), Article number: 100285. DOI: 10.1016/j.crmicr.2024.100285

7. Lesk C., Rowhani P., Ramankutty N. Influence of extreme weather disasters on global crop production // Nature. 2016. Vol. 529, Article number: 7584. P. 84–87. DOI: 10.1038/nature16467.

8. Lin W., Guo X., Pan X., Li Z. Chlorophyll composition, chlorophyll fluorescence, and grain yield change in esl mutant rice //International Journal of Molecular Sciences. 2018. Vol. 19(10), Article number: 2945.

9. Makino A., Osmond B. Solubilization of ribulose-1, 5-bisphosphate carboxylase from the membrane fraction of pea leaves // Photosynthesis research. 1991. Vol. 29, P. 79–85. DOI: 10.1007/BF00035378

10. Matiu M., Ankerst D. P., Menzel A. Interactions between temperature and drought in global and regional crop yield variability during 1961-2014 // PloS one. 2017. Vol. 12(5), Article number: e0178339. DOI: 10.1371/journal.pone.0178339

11. Michaletti A., Naghavi M. R., Toorchi M., Zolla L., Rinalducci S., Michaletti A. et al. Metabolomics and proteomics reveal drought-stress responses of leaf tissues from spring-wheat // Scientific reports. 2018. Vol. 8(1), Article number: 5710. DOI: 10.1038/s41598-018-24012-y

12. Noor H., Sun M., Algwaiz H. I. M. et al. Chlorophyll fluorescence and grain filling characteristic of wheat (Triticum aestivum L.) in response to nitrogen application level // Molecular Biology Reports. 2022. Vol. 49(7), P. 7157–7172. DOI: 10.1007/s11033-022-07612-w

13. Sugiura D., Mitsuya S., Takahashi H., Yamamoto R., Miyazawa Y. Microcontroller-based water control system for evaluating crop water use characteristics // Plant Methods. 2024. Vol. 20(1), Article number: 179. DOI: 10.1186/s13007-024-01305-0

14. Tambussi E. A., Maydup M. L., Carrión C. A., Guiamet J. J., Araus J. L. Ear photosynthesis in C3 cereals and its contribution to grain yield: methodologies, controversies, and perspectives // Journal of Experimental Botany. 2021. Vol. 72(11), P. 3956–3970. DOI: 10.1093/jxb/erab125

15. Thenappan D. P., Pandey R., Hada A., Jaiswal D. K., Chinnusamy V., Bhattacharya R., Annapurna K. Physiological Basis of Plant Growth Promotion in Rice by Rhizosphere and Endosphere Associated Streptomyces Isolates from India // Rice (N Y). 2024. Vol. 17(1), Article number: 60. DOI: 10.1186/s12284-024-00732-w

16. Zhu D., Zhu G., Zhang Z., Wang Z., Yan X., Yan Y., Zhu D. et al. Effects of independent and combined water-deficit and high-nitrogen treatments on flag leaf proteomes during wheat grain development // International Journal of Molecular Sciences. 2020. Vol. 21(6), Article number: 2098. DOI: 10.3390/ijms21062098