Estimation of the development of androgenic dihaploid lines of winter wheat of the R1 generation in vivo
https://doi.org/10.31367/2079-8725-2024-94-5-26-32
Abstract
Development of dihaploid lines using biotechnological methods can reduce not only the time required to breed a new variety, but also labor costs and technical resources. Earlier in our studies, there has been obtained seed material of androgenic dihaploid of the regenerated plants of the R0 generation, grown ex vitro. The next stage of the work delt with sowing the seeds of these plants in vivo. The purpose of the current study was to estimate the development of winter wheat plants of androgenic dihaploid lines of the R1 generation and parental varieties in vivo according to such characteristics as a length of phenophases, a vegetation period, morphometric estimation for comparison with each other and with parental genotypes, as well as for seed propagation. As a result of the study carried out in 2022–2024, there has been estimated a new nitial material of winter common wheat obtained on the basis of previously conducted biotechnological work. There have been studied androgenic dihaploid R1 lines according to the traits of vegetative and generative organs, for grain productivity of plants and seed germination. There have been selected promising lines for further breeding evaluation. Androgenic dihaploid lines of winter wheat of the R1 generation 79.1.5.1, 79.1.5.2, 79.2.1.1, 84.3.1.1, 84.3.3.1, 85.2.1.1 were the best according to vegetative, generative traits, grain productivity and seed germination. The best indicators of productivity and germination were characterized by androgenic dihaploid lines based on the hybrid ‘Rostovchanka 7 x Bezostaya 100’.
About the Authors
N. V. KalininaRussian Federation
researcher of the laboratory for cell breeding
347740, Rostov region, Zernograd, Nauchny Gorodok Str., 3; e-mail: vniizk30@mail.ru
N. G. Chertkova
Russian Federation
junior researcher of the laboratory for cell breeding
347740, Rostov region, Zernograd, Nauchny Gorodok Str., 3; e-mail: vniizk30@mail.ru
V. Yu. Dontsova
Russian Federation
junior researcher of the laboratory for cell breeding
347740, Rostov region, Zernograd, Nauchny Gorodok Str., 3; e-mail: vniizk30@mail.ru
D. M. Marchenko
Russian Federation
Candidate of Agricultural Sciences, leading researcher of the department of winter wheat breeding and seed production
347740, Rostov region, Zernograd, Nauchny Gorodok Str., 3; e-mail: vniizk30@mail.ru
References
1. Zhangaziev A.S., Taichibekov A.U., Kashkynbaeva L.B., Dyusenbaeva Zh. S. Gaploidnaya tekhnologiya v ispol'zovanii metodov in vitro v selektsii ozimoi pshenitsy [Haploid technology in the use of in vitro methods in winter wheat breeding] // Uspekhi sovremennogo estestvoznaniya. 2016. № 7. S. 42–45. EDN ZDGVPT.
2. Kruglova N.N., Sel'dimirova O. A., Zaitsev D. Yu., Galin I.R., Zinatullina A.E., Anokhina N.S. Razvitie androklinnykh rastenii pshenitsy v polevykh usloviyakh in vivo [Development of androclinal wheat plants in field conditions in vivo] // Izvestiya Ufimskogo nauchnogo tsentra RAN. 2017. № 3. S. 26–30.
3. Kuperman, F.M. Morfofiziologiya rastenii: Morfofiziologicheskii analiz etapov organogeneza razlichnykh zhiznennykh form pokrytosemennykh rastenii: ucheb. posobie dlya biol. spetsial'nostei vuzov [Morphophysiology of plants: Morphophysiological analysis of the stages of organogenesis of various life forms of angiosperms] / 3-e izd., dop. Moskva: Vysshaya shkola, 1977. 288 s.
4. Sel'dimirova O. A., Kruglova N.N. Androklinnyi embrioidogenez in vitro zlakov [Androclinal embryogenesis in vitro of cereals] // Uspekhi sovremennoi biologii. 2014. T. 134, № 5. S. 476–487.
5. Sozinova, L.F. Rasshirenie genofonda myagkoi pshenitsy na Severe Kazakhstana metodami biotekhnologii. Itogi 2004 goda [Expansion of the gene pool of common wheat in the North of Kazakhstan by biotechnology methods. Results of 2004] // Biotekhnologiya. Teoriya i praktika. 2005. № 1. S. 51–55.
6. Chertkova N.G., Kalinina N.V., Dontsova V.Yu. Marchenko D.M. Otsenka rezul'tativnosti polucheniya regenerantnykh linii ozimoi myagkoi pshenitsy v kul'ture pyl'nikov in vitro [Estimation of the efficiency of obtaining regenerant lines of winter common wheat in the culture of anther in vitro] // Zernovoe khozyaistvo Rossii. 2023. T. 15, № 1. S. 76–81. DOI: 10.31367/2079-8725-2023-84-1-76-81
7. Osadchaya T.S., Trubacheeva N.V., Kravtsova L.A., Belan I.A., Rosseeva L.P., Pershina L.A. Izuchenie fertil'nosti i tsitogeneticheskoi izmenchivosti u androgennykh rastenii (R0 i R1) alloplazmaticheskikh introgressivnykh linii myagkoi pshenitsy [Study of fertility and cytogenetic variability in androgenic plants (R0 and R1) of alloplasmic introgressive lines of common wheat] // Vavilovskii zhurnal genetiki i selektsii. 2016. T. 20, № 3. S. 370–377. DOI: 10.18699/VJ16.165
8. Grauda D., Lepse N., Strazdina V., Kokina I., Lapina L., Mikelsone A., Lubinskis L., Rashal I. Obtaining of doubled haploid lines by anther culture method for the Latvian wheat breeding // Agronomy Research. 2010. Vol. 8, № 3. P. 545–552.
9. Grauda D., Zagata K., Lanka G., Strazdina V., Fetere V., Lisina N., Krasnevska N., Fokina O., Mikelsone A., Ornicans R., Belogrudova I., Rashal I. Genetic diversity of wheat (Triticum aestivum L.) plants-regenerants produced by anther culture // Vavilov Journal of Genetics and Breeding. 2016. Vol. 20, № 4. P. 537–544. DOI: 10.18699/VJ16.176
Review
For citations:
Kalinina N.V., Chertkova N.G., Dontsova V.Yu., Marchenko D.M. Estimation of the development of androgenic dihaploid lines of winter wheat of the R1 generation in vivo. Grain Economy of Russia. 2024;16(5):26-32. (In Russ.) https://doi.org/10.31367/2079-8725-2024-94-5-26-32