Selection of molecular markers for genetic certification of Triticum aestivum
M. A. Samarina,
D. S. Uliyanov,
T. D. Mokhov,
Ya. S. Meglitskaya,
P. Yu. Krupin,
G. I. Karlov,
P. N. Kharchenko,
S. I. Voronov,
N. V. Davydova,
M. G. Divashuk https://doi.org/10.31367/2079-8725-2024-93-4-33-40
Abstract
According to the Law “On Seed Production”, all varieties or hybrids included in the state register shall be provided for genetic passports, as well as making a list of plants’ species, the production of which is aimed at ensuring food security. However, the use of morphological characteristics does not allow distinguishing genetically similar samples, identifying hidden genetic variability and ensuring control of the homogeneity of the initial material. In connection with these limitations, the purpose of the current work was to develop approaches for the selection of molecular genetic mic- rosatellite markers (SSR markers) to differentiate common wheat varieties. In accordance with the purpose, there has been conducted a literature review regarding methods for genetic certification and evaluation of the diversity of spring common wheat based on polymorphism of microsatellite loci, and there has been generated a set of Triticum aestivum genome assemblies from the NCBI Gen Bank. Based on the literature data, there have been selected the most polymorphic SSR markers using the invented algorithm. The analysis of databases of SSR markers in the genome of common wheat using bioinformatics methods allowed establishing a minimal discriminatory set of 20 markers that can detect 419 different alleles in Triticum aestivum. The results obtained can become the necessary theoretical foundation for further practical research.
About the Authors
M. A. Samarina
FSBSI “All-Russian Research Institute of Agricultural Biotechnology” (FSBSI ARRIAB); FSBSI “Federal Research Center “Nemchinovka” (FRC “Nemchinovka”)
Russian Federation
Russian Federation
junior researcher of the laboratory for genetic technologies and molecular support of breeding of grain crops and legumes; junior researcher of the laboratory for genetics and pre-breeding,
127550, Moscow, Timiryazevskaya Str., 42;
143026, Moscow region, Odintsovsky region, Odintsovsky district, v. of Novoivanovskoe, Agrokhimikov Str., 6.
D. S. Uliyanov
FSBSI “All-Russian Research Institute of Agricultural Biotechnology” (FSBSI ARRIAB); FSBSI “Federal Research Center “Nemchinovka” (FRC “Nemchinovka”)
Russian Federation
Russian Federation
junior researcher of the laboratory for digital phenotyping for plant breeding; junior researcher of the laboratory for genetics and pre-breeding,
127550, Moscow, Timiryazevskaya Str., 42;
143026, Moscow region, Odintsovsky region, Odintsovsky district, v. of Novoivanovskoe, Agrokhimikov Str., 6.
T. D. Mokhov
FSBSI “All-Russian Research Institute of Agricultural Biotechnology” (FSBSI ARRIAB); FSBSI “Federal Research Center “Nemchinovka” (FRC “Nemchinovka”)
Russian Federation
Russian Federation
junior researcher of the laboratory for digital phenotyping for plant breeding; junior researcher of the laboratory for genetics and pre-breeding,
127550, Moscow, Timiryazevskaya Str., 42;
143026, Moscow region, Odintsovsky region, Odintsovsky district, v. of Novoivanovskoe, Agrokhimikov Str., 6.
Ya. S. Meglitskaya
FSBSI “All-Russian Research Institute of Agricultural Biotechnology” (FSBSI ARRIAB); FSBSI “Federal Research Center “Nemchinovka” (FRC “Nemchinovka”)
Russian Federation
Russian Federation
junior researcher of the laboratory for digital phenotyping for plant breeding; junior researcher of the laboratory for genetics and pre-breeding,
127550, Moscow, Timiryazevskaya Str., 42;
143026, Moscow region, Odintsovsky region, Odintsovsky district, v. of Novoivanovskoe, Agrokhimikov Str., 6.
P. Yu. Krupin
FSBSI “All-Russian Research Institute of Agricultural Biotechnology” (FSBSI ARRIAB); FSBSI “Federal Research Center “Nemchinovka” (FRC “Nemchinovka”)
Russian Federation
Russian Federation
Candidate of Biological Sciences, head of the laboratory for genetic technologies and molecular support of breeding of grain crops and legumes; junior researcher of the laboratory for genetics and pre-breeding,
127550, Moscow, Timiryazevskaya Str., 42;
143026, Moscow region, Odintsovsky region, Odintsovsky district, v. of Novoivanovskoe, Agrokhimikov Str., 6.
G. I. Karlov
FSBSI “All-Russian Research Institute of Agricultural Biotechnology” (FSBSI ARRIAB)
Russian Federation
Russian Federation
Doctor of Biological Sciences, Academician of RAS, Professor, Head,
127550, Moscow, Timiryazevskaya Str., 42.
P. N. Kharchenko
FSBSI “All-Russian Research Institute of Agricultural Biotechnology” (FSBSI ARRIAB)
Russian Federation
Russian Federation
Doctor of Biological Sciences, Academician of RAS, Professor, Head of the Department of Cell and Gene Engineering; Scientific Supervisor,
127550, Moscow, Timiryazevskaya Str., 42
S. I. Voronov
FSBSI “Federal Research Center “Nemchinovka” (FRC “Nemchinovka”)
Russian Federation
Russian Federation
Doctor of Biological Sciences, Correspondent Member of the RAS, Professor, Head,
143026, Moscow region, Odintsovsky region, Odintsovsky district, v. of Novoivanovskoe, Agrokhimikov Str., 6.
N. V. Davydova
FSBSI “Federal Research Center “Nemchinovka” (FRC “Nemchinovka”)
Russian Federation
Russian Federation
Doctor of Agricultural Sciences, Head of the Laboratory for Breeding and Primary Seed Production of Spring Wheat,
143026, Moscow region, Odintsovsky region, Odintsovsky district, v. of Novoivanovskoe, Agrokhimikov Str., 6
M. G. Divashuk
FSBSI “All-Russian Research Institute of Agricultural Biotechnology” (FSBSI ARRIAB); FSBSI “Federal Research Center “Nemchinovka” (FRC “Nemchinovka”)
Russian Federation
Russian Federation
Candidate of Biological Sciences, Leading Researcher, Head of the Laboratory for Genetics and Pre-breeding; Head of the Kurchatov Genome Center,
127550, Moscow, Timiryazevskaya Str., 42;
143026, Moscow region, Odintsovsky region, Odintsovsky district, v. of Novoivanovskoe, Agrokhimikov Str., 6.
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Review
For citations:
Samarina M.A., Uliyanov D.S., Mokhov T.D., Meglitskaya Ya.S., Krupin P.Yu., Karlov G.I., Kharchenko P.N., Voronov S.I., Davydova N.V., Divashuk M.G. Selection of molecular markers for genetic certification of Triticum aestivum. Grain Economy of Russia. 2024;16(4):33-40. (In Russ.) https://doi.org/10.31367/2079-8725-2024-93-4-33-40
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