BREEDING OF RICE VARIETIES WITH PYRAMIDING GENES TOLERANT TO DISEASES

Rice can significantly decrease the harvest of grain when it’s damaged with a dangerous fungus ‘Pyricularia grisea’ (rice blast). Single genes resistant to the pathogen are usually unefficient. That’s why it’s necessary to breed productive rice varieties, resistant to rice blast, having several genes possessing resistance in one genotype. Using molecular markers closely connected with genes which make the plants resistant to the pathogen, significantly facilitates the breeding. The purpose of the work is to breed a line of rice with five genes resistant to rice blast using a molecular marking method.  We used the lines of foreign breeding as donors of resistant genes and domestic varieties ‘Boyarin’ and ‘Virazh’ as recipients. In the work there were used micro satellite markers on genes resistant to rice blast. The results were visualized by polymerase chain reaction (PCR) and electrophoresis.

At the first stage we received 6 hybrids from a cross breeding of recipients ‘Boyarin’ and ‘Virazh’ and donors Pi-l, Pi-2, Pi-33. At the second stage during the process of pyramiding we managed to receive the varieties with these three genes together. At the third stage we carried out the hybridization with donors Pi-ta and Pi-b to combine 5 genes.

As a result of long-term work in introgression of the genes resistant to rice blast with the help of marking selection and PCR-analysis we obtained rice lines, combining five genes Pi-l, Pi-2, Pi-33, Pi-ta, Pi-b resistant to the pathogen. 

1. 1. Correa-Victoria, F.J. Gene combinations in rice for the development of durable resistance to Pyricularia grisea in Colombia / Correa-Victoria F.J., Tharreau D., Martinez C., Vales M. et al. // Proc. 3rd Int. Temperate Rice Conference, Punta del Este, 2003.

2. Silue, D. Evidence for a gene-for-gene relationship in the Oryza sativa - Magnaporthe grisea pathosystem/ Silue, D., Notteghem J.L., Tharreau D. // Phytopathology, 1992. –82. – Р.577-580.

3. Chao, C.T. Genetic analysis of resistance/susceptibility in individual F3 families of rice against strains of Magnaporthe grisea containing different genes for avirulence/ C.T. Chao, K.A. Moldenhauer, A.H. Ellingboe // Euphytica, 1999. –109. – Р.183-190.

4. Mackill, D.J. Inheritance of blast resistance in near-isogenic lines of rice/ D.J.Mackill, J.M. Bonman // Phytopathology, 1992. –82. – Р.746-749.

5. Yu, Z.H. Molecular mapping of genes for resistance to rice blast (Pyricularia grisea Sacc.)/ Z.H. Yu, D.J. Mackill, J.M. Bonman, S.R. McCouch, E. Guideroni, J.L. Notteghem, S.D. Tanksley// Theor. Appl. Genet., 1996. – 93. – Р.859-863.

6. Bryan, G.T. A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta/ G.T. Bryan, K. Wu, L. Farrall, Y. Jia, H.P. Hershey, S. McAdams, R. Tarchini, G. Donaldson, K. Faulk, B. Valent// Plant Cell, 2000. – 12. – Р.2033-2045.

7. Inukai, T. Allelism of blast resistance genes in near-isogenic lines of rice/ T. Inukai, R.J. Nelson, R.S. Zeigler, S. Sarkarung, D.J. Mackill, J.M. Bonman, T. Takamure, T. Kinoshita // Phytopathology, 1994. – 84. – Р.1278-1283.

8. Wang, Z.X. The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes / Z.X. Wang, M. Yano, U.Yamanouchi, M.Iwamoto, L. Monna, H. Hayasaka, Y. Katayose, T. Sasaki// Plant J., 1999. – 19. – Р.55-64.

9. Wise, R.P. Disease resistance: What’s brewing in barley genomics/ R.P. Wise // Plant Dis., 2000. – 84. – Р.1160-1170.

10. Jena, K.K. Marker assisted selection – a new paradigm in plant breeding/ K.K. Jena, H.P. Moon, D.J. Mackill // Korean J. Breed., 2003. – V.35. – P. 133-140.

11. Khavkin, E.E. Molecular plant breeding: DNA-technologies of breeding of new crops/ Е.Е. Khavkin // Agricultural biology, 2003. - №3. – P. 26-41.

12. Kostylev, P.I. Combination of five genes tolerant to rice blast in one genotype with the help of DNA-markers/ P.I. Kostylev, E.V. Krasnova, A.A. Redkin, Zh.M. Mukhina, E.V. Dubina// The VIII-th International science practical conference “Biological protection of plants as a basis of agro eco system stabilization”, Krasnodar, 2014. – P. 25-28.

13. Kostylev, P.I. Transfer of five genes tolerant to rice blast with the help of DNA-markers/ P.I. Kostylev, P.I. Shilov, Zh.M. Mukhina //Vestnik of RAAS, 2014. – №1. – P.33-35.