The study of the efficiency of anther culture in vitro of bread wheat varieties and hybrids (Triticum aestivum L.)
https://doi.org/10.31367/2079-8725-2022-83-6-17-22
Abstract
Anther culture is the most effective and not technically complex tool for obtaining double wheat haploids. The current study is devoted to the research of the influence of the genotype and concentration of the growth regulator 2.4-D on the efficiency of androgenesis in vitro of spring bread wheat. The purpose of the study was to estimate the efficiency of the anther culture of hybrids of the first and second generation and their parental varieties and to study its dependence on different concentrations of the growth regulator 2.4-D. There has been carried out the estimation of the traits of the F1 hybrids, the F2 population, and the parental varieties ‘Obskaya 2’ and ‘Novosibirskaya 15’ when 2.4-D was added to the induction medium at a concentration of 1 and 2 mg/l. There has been established that the F2 population and the parental variety ‘Novosibirskaya 15’ are characterized by the largest indicators. The average values were significantly surpassed by the F2 population in the traits ‘number of productive anthers per 100 anthers’ and ‘number of all regenerants per 100 anthers’ at 1 mg/l of 2.4-D. The variety ‘Novosibirskaya 15’ surpassed the average values in traits ‘number of sprouts per 100 anthers’ and ‘number of all regenerants per 100 sprouts’ at 2 mg /l of 2.4-D. The trial has shown that the best responsiveness of the anther culture for the studied genotypes was when the growth regulator 2.4-D was added to the induction medium at a concentration of 1 mg/l. The variety ‘Novosibirskaya 15’ has been characterized by good responsiveness in anther culture in vitro and could be used as a donor of valuable alleles in wheat breeding programs using DH technologies.
About the Authors
N. V. Petrash
SibRIPGB, a branch of the FRCIC&G SibB of the RAS
Russian Federation
Russian Federation
N.V. Petrash, junior researcher of the laboratory for plant gene pool
630501, Novosibirsk region, Novosibirsk, v. of. Krasnoobsk, S-100, bld. 21, PO Box 375
Е. А. Orlova
SibRIPGB, a branch of the FRCIC&G SibB of the RAS
Russian Federation
Russian Federation
Е. А. Orlova, Candidate of Agricultural Sciences, leading researcher of the laboratory for plant gene pool
630501, Novosibirsk region, Novosibirsk, v. of. Krasnoobsk, S-100, bld. 21, PO Box 375
I. Е. Likhenko
SibRIPGB, a branch of the FRCIC&G SibB of the RAS
Russian Federation
Russian Federation
I. Е. Likhenko, Doctor of Agricultural Sciences, head of the laboratory for breeding, seed production and cultivation technologies of field crops
630501, Novosibirsk region, Novosibirsk, v. of. Krasnoobsk, S-100, bld. 21, PO Box 375
V. V. Piskarev
SibRIPGB, a branch of the FRCIC&G SibB of the RAS
Russian Federation
Russian Federation
V. V. Piskarev, Candidate of Agricultural Sciences, head of the laboratory for plant gene pool
630501, Novosibirsk region, Novosibirsk, v. of. Krasnoobsk, S-100, bld. 21, PO Box 375
References
1. Kruglova N. N., Sel'dimirova O. A. Sravnitel'naya otsenka chastoty obrazovaniya androklin-nykh embrioidov u roditel'skikh sortov, gibridov F1 i digaploidnykh linii gibridov F1 yarovoi myagkoi pshenitsy [Comparative estimation of the frequency of formation of androclinic embryoids in parental varieties, F1 hybrids and dihaploid lines of F1 hybrids of spring bread wheat] // Permskii agrarnyi vestnik. 2015. № 2 (10). S. 66–71.
2. Nekrasova O. A., Kalinina N. V. Faktory, vliyayushchie na protsessy androgeneza pri kul'tivirovanii pyl'nikov pshenitsy (obzor) [Factors influencing the androgenesis processes during wheat anthers’ cultivation (review)] // Zernovoe khozyaistvo Rossii. 2022. T. 14, № 3. S. 25–30. DOI: 10.31367/2079-8725-2022-81-3-25-30.
3. Pershina L. A., Osadchaya T. S., Badaeva E. D., Belan I. A., Rosseeva L. P. Izuchenie osobennostei androgeneza v kul'ture pyl'nikov sortov i perspektivnoi formy yarovoi myagkoi pshenitsy zapadnosibirskoi selektsii, razlichayushchikhsya nalichiem ili otsutstviem pshenichno-chuzherodnykh translokatsii [Study of the characteristics of androgenesis in the anthers culture of varieties and a promising form of spring bread wheat of West Siberian breeding, differing in the presence or absence of wheat-alien translocations] // Vavilovskii zhurnal genetiki i selektsii. 2014. T. 17, №. 1. S. 40–49.
4. Polunin D. A., Shtaiger I. A., Efimov V. M. Razrabotka programmnogo kompleksa JACOBI 4 dlya mnogomernogo analiza mikrochipovykh dannykh [Development of the JACOBI 4 software package for multivariate analysis of microchip data] // Vestnik Novosibirskogo gosudarstvennogo universiteta. Seriya: Informatsionnye tekhnologii. 2014. T. 12, Vyp. 2. S. 90–98.
5. Timonova E. M., Adonina I. G., Salina E. A. Izuchenie vliyaniya chuzherodnykh translokatsii na pokazateli androgeneza in vitro u linii myagkoi pshenitsy (Triticum aestivum L.) [Study of the effect of alien translocations on the androgenesis’ parameters in vitro in the bread wheat lines (Triticum aestivum L.)] // Trudy po prikladnoi botanike, genetike i selektsii. 2022. T. 183, №. 1. S. 127–134.
6. Castillo A. M., Sánchez-Díaz R. A., Vallés M. P. Effect of ovary induction on bread wheat anther culture: ovary genotype and developmental stage, and candidate gene association // Frontiers in Plant Science. 2015. Vol. 6. P. 402. DOI: 10.3389/fpls.2015.00402.
7. El-Fatah A., Bahaa E. S., Sayed M. A., El-Sanusy S. A. Genetic analysis of anther culture response and identification of QTLs associated with response traits in wheat (Triticum aestivum L.) // Molecular Biology Reports. 2020. Vol. 47, № 12. P. 9289–9300. DOI: 10.1007/s11033-020-06007-z.
8. Lantos C., Pauk J. Factors influencing the efficiency of wheat anther culture // Acta Biologica Cracoviensia. Series Botanica. 2020. Vol. 62, № 2. P. 7–16. DOI: 10.24425/abcsb.2020.131671.
9. Lazaridou T., Pankou C., Xynias I., Roupakias D. Effect of D genome on wheat anther culture response after cold and mannitol pretreatment // Acta Biologica Cracoviensia s. Botanica. 2016. Vol. 58, № 1. DOI: 10.1515/abcsb-2016-0006.
10. Nielsen N. H., Andersen S. U., Stougaard J., Jensen A., Backes G., Jahoor A. Chromosomal regions associated with the in vitro culture response of wheat (Triticum aestivum L.) microspores // Plant breeding. 2015. Vol. 134, № 3. S. 255–263. DOI: 10.1111/pbr.12257.
Review
For citations:
Petrash N.V., Orlova Е.А., Likhenko I.Е., Piskarev V.V. The study of the efficiency of anther culture in vitro of bread wheat varieties and hybrids (Triticum aestivum L.). Grain Economy of Russia. 2022;(6):17-22. (In Russ.) https://doi.org/10.31367/2079-8725-2022-83-6-17-22
Views:
342
Email this article 