Изучение биоразнообразия PYRICULARIA ORYZAE CAV. в рисосеющих зонах юга России на основе методов ПЦР

The study of biodiversity of Pyricularia oryzae Cav. in the rice-growing areas of the south of Russia using the methods of PCR On the basis of molecular-genetic approaches there has been conducted the study of biodiversity of phyto pathogenic fungus Pyricularia oryzae Cav. in the south of Russia. We have conducted the monitoring and selected the isolates of the pathogen from the infected herbarium material, collected from the fields of six rice-growing areas of the Krasnodar Kray with various degree of harmfulness of rice blast pathogen. Their DNA-passports have been made. The studied isolates of the blast pathogen have been characterized in respect of their morphological traits. The phyto-pathological test with the application of varieties-differentiators of rice has established quantitative and qualitative composition of the virulent genes in fungus strains. The study has found the genes of pathogen tolerance, greatly efficient in the south of Russia, which can be recommended for the breeding programs for growing rice varieties resistant to rice blast.

рис, пирикуляриоз, изоляты, ПЦР, микросателлитные маркеры, ДНК-паспорта, гены (а)вирулентности, rice, blast, isolates, PCR, microsatellite markers, DNA-passports, virulent genes

1. Kovalenko, E.D. Methodical guidelines for assessing the resistance of rice cultivars to the causative agent of pyriculyariosis / E.D. Kovalenko, Yu.V. Gorbunova, A.A. Kovaleva and others - M., 1988. - 30 p.

2. Castiglioni P., Pozzi C., Heun M., Terzi V., Muller K. J., Rohde W., Salamini F. An AFLP-Based Procedure for the Efficient Mapping of Mutations and DNA Probes in Barley // Genetics.- 1998.- V. 149.- P. 2039-2056.

3. Kumar, J., R. и Zeigler (1995): Mating behaviour of Magnaporthe grisea from Central Himalayas of India. Phytopathology 85, 1201(Abstract).

4. Padmanabhan, S. Y., N. K. Chakrabarti, S. C. Mathur, J. Veerar-aghwan (1970): Identification of pathogenic races of Pyricularia o-ryzae in India. Phytopathology 60, 1574-1577.

5. Resurreccion A.P., Villareal C.P., Parco A., Second G., Juliano B.O. Classification of cultivated rices into indica and japonica types by the izozyme, RFLP, and two milled-rice methods // Theor. Appl. Genet.- 1994.- V.89.- P. 14-18.

6. Ahn S.N., Kim Y.K., Han S.S., Choi H.C., Moon H.P., McCouch S.R. Molecular mapping of a gene for resistance to a Korean isolate of rice blast // Rice Genet. Newsl.- 1996.- V.13.- P. 74-76.

7. Bryan G.T., Wu K.S., Farrall L., Jia Y., Hershley H.P. et al. tA single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta // The Plant Cell.- 2000.- V.12.- P. 2033-2045.

8. Lang N.T., Khush G.S., Huang N., Buu B. Fine mapping for blast resistance gene in rice (Oryza sativa L.) using bulked segregant analysis // Omonrice.- 2001.-V.1.- P. 1-8.

9. Launder E.S., Green P., Abrahamson J., Barlow A., Daly M.J., Lincoln S.E., Newburg L. MAPMAKER: An interactive computer package for constructing primary genetic linkage map of experimental and natural population // Genomics.- 1987.- V.1.- P. 174-181.

10. Arase S., Fujita K., Uehara T., Honda Y. and Isota J. Light-Enhanced Resistance to Magnaporthe grisea Infection in the Rice Sekiguchi Lesion Mutants // Journal of Phytopathology. - 2000. - Vol. 148. - P. 197-203.

11. Ayres N. M., McClung A. M., Larkin H. P. D. Bligh H.F. J., Jones C. A., Park W. D. Microsatellites and a single-nucleotide polymorphism differentiate apparent amylose classes in an extended pedigree of US rice germ plasm // Theor. Appl. Genet.-1997.- V.94.- P. 773-781.

12. Jensen L.B., Courtous B., Shen L., Li Z., et al. Locating genes controlling allelopathic effects against barnyardgrass in upland rice // Agron. Journ.- 2001.- V.93.- P. 21-26.

13. Kaye C., Milazzo J., Rozenfeld S., Lebrun M.-H., Tharreau D. The development of simple sequence repeat (SSR) markers for Magnaporthe grisea and their integration into an established molecular linkage map // Fungal Genet Biol. - 2003. - Vol. 40 (3). - P. 207-14.

14. Conaway-Bormans C.A., Marchetti M.A., Johnson C.W., McClung A.M., Park W.D. Molecular markers linked to the blast resistance gene Pi-z, in rice for use in marker-assisted selection // Theor. Appl. Genet.-2003.- V.107.- P. 1014-1020.;

15. Fuji K., Hayano-Saito Y., Sugiura N., Hayashi N., Saka N. et al. Gene analysis of panicle blast resistance in rice cultivars with rice stripe resistance // Breed. Res. 1999.- V.1.- P. 203-210.

16. Xia, J. Q., J. C. Corell, F. N., Lee, M. A. Marchetti, D. D. Rhoads (1993): DNA fingerprinting to examine microgeographic variation in Magnaporthe grisea (Pyricularia grisea) population in two rice fields in Arkansas. Phytopathology 83, 1029-1035

17. Levy, M., F. J. Correa-Victoria, R. S. Zeigler, S. Shizong, J. E. Hamer (1993): Genetic diversity of rice blast fungus in a disease nursery in Colombia. Phytopathology 83, 1427-1433.

18. Khush G.S., Brar D.S., Hardy B. Rice genetics VI.- Los Banos. 2001.- 488 p.

19. Murray M.G., Thompson W.F. Rapid isolation of high molecular weight plant DNA // Nucleic Acids Research. - 1980. - №10. - Р. 4321-4325.

20. Adreit, H. Microsatellite markers for population studies of the rice blast fungus, Magnaporthe grisea./ H. Adreit, D. Santoso, D. Andriantsimialona, W. Utami, J.L. Notteghem, M.H. Lebrun and D. Tharreau.// Journal compilation Molecular Ecology Notes, 2007, Р. 667-670.

21. Li C. Y., Li J.B., LiuU L., Yang J., Su Y., Wang Y. Y., Xie Y., Ye M. and Zhu Y. Y. Development of minisatellite markers in phytopathogenic fungus, Magnaporthe grisea // Molecular Ecology Notes - 2007. - Vol.131. - P. 144-152.

22. Ahn S.N., Kim Y.K., Han S.S., Choi H.C., Moon H.P., McCouch S.R. Molecular mapping of a gene for resistance to a Korean isolate of rice blast // Rice Genet. Newsl.- 1996.- V.13.- P. 74-76.

23. Kaye, C., Milazzo, J., Rozenfeld, S., Lebrun, M.H., Tharreau, D., 2003. The development of simple sequence repeat markers for Magnaporthe grisea and their integration into an established genetic linkage map. Fungal Genet. Biol. 40, 207-214.

24. Usatov A.V., Kostylev P.I., Azarin K.V., Markin N.V., Makarenko M.S., Khachumova V.A., Bibov M.Yu. Introgression of the rice blast resistance genes Pi1, Pi2 and Pi33 into Russian rice varieties by marker-assisted selection / Indian Journal of Genetics and Plant Breeding. 2016. - Т. 76. - № 1. - С.18-23.

25. Костылев П.И. Магнат - первый отечественны сорт риса, созданный на основе MAS-селекции / П.И. Костылев, Е.В. Краснова, А.А. Редкин // Сборник материалов Международной научно-практической конференции «Биотехнологии в комплексном развитии регионов», 15-17 марта 2016 г., Москва.

26. Костылев, П.И. Пентаген - новый сорт риса с пятью генами устойчивости к пирикуляриозу, созданный с помощью маркерной селекции / П.И. Костылев // Сборник Международной научно-практической конференции «Фундаментальные и прикладные исследования в биоорганическом сельском хозяйстве России, СНГ и ЕС» 9-12 августа 2016 г. Том №2. С.113-121.