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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">zhros</journal-id><journal-title-group><journal-title xml:lang="ru">Зерновое хозяйство России</journal-title><trans-title-group xml:lang="en"><trans-title>Grain Economy of Russia</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2079-8725</issn><issn pub-type="epub">2079-8733</issn><publisher><publisher-name>Федеральное государственное бюджетное научное учреждение "Аграрный научный центр "Донской»</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.31367/2079-8725-2023-88-5-48-55</article-id><article-id custom-type="elpub" pub-id-type="custom">zhros-2463</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>СЕЛЕКЦИЯ И СЕМЕНОВОДСТВО СЕЛЬСКОХОЗЯЙСТВЕННЫХ РАСТЕНИЙ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>PLANT BREEDING AND SEED PRODUCTION OF AGRICULTURAL CROPS</subject></subj-group></article-categories><title-group><article-title>Оценка суходольных образцов риса на присутствие гена засухоустойчивости qDTY1.1 с помощью ДНК-маркера</article-title><trans-title-group xml:lang="en"><trans-title>Estimation of upland rice samples for the presence of the drought resistance gene qDTY1.1 using a DNA marker</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-4371-6848</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Костылев</surname><given-names>П. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Kostylev</surname><given-names>P. I.</given-names></name></name-alternatives><bio xml:lang="ru"><p>доктор сельскохозяйственных наук, главный научный сотрудник лаборатории селекции и семеноводства риса</p><p>347740, Ростовская обл., г. Зерноград, ул. Научный городок, д. 3</p></bio><bio xml:lang="en"><p>Doctor of Agricultural Sciences, senior researcher of the laboratory for rice breeding and seed production</p><p>347740, Rostov region, Zernograd, Nauchny Gorodok Str., 3</p></bio><email xlink:type="simple">p-kostylev@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-2046-4000</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Вожжова</surname><given-names>Н. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Vozhzhova</surname><given-names>N. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>кандидат сельскохозяйственных наук, старший научный сотрудник ЦФНИ</p><p>347740, Ростовская обл., г. Зерноград, ул. Научный городок, д. 3</p></bio><bio xml:lang="en"><p>Candidate of Agricultural Sciences, senior researcher of the CFRI</p><p>347740, Rostov region, Zernograd, Nauchny Gorodok Str., 3</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-6641-878X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Аксенов</surname><given-names>А. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Aksenov</surname><given-names>A. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>младший научный сотрудник лаборатории селекции и семеноводства риса</p><p>347740, Ростовская обл., г. Зерноград, ул. Научный городок, д. 3</p></bio><bio xml:lang="en"><p>junior researcher of the laboratory for rice breeding and seed production</p><p>347740, Rostov region, Zernograd, Nauchny Gorodok Str., 3</p></bio><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ФГБНУ «Аграрный научный центр «Донской»</institution><country>Россия</country></aff><aff xml:lang="en"><institution>FSBSI Agricultural Research Center «Donskoy»</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2023</year></pub-date><pub-date pub-type="epub"><day>09</day><month>11</month><year>2023</year></pub-date><volume>0</volume><issue>5</issue><fpage>48</fpage><lpage>55</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Костылев П.И., Вожжова Н.Н., Аксенов А.В., 2023</copyright-statement><copyright-year>2023</copyright-year><copyright-holder xml:lang="ru">Костылев П.И., Вожжова Н.Н., Аксенов А.В.</copyright-holder><copyright-holder xml:lang="en">Kostylev P.I., Vozhzhova N.N., Aksenov A.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.zhros.online/jour/article/view/2463">https://www.zhros.online/jour/article/view/2463</self-uri><abstract><p>Засуха является основным стресс-фактором при выращивании растений. Воздействие засухи на растения риса проявляется от уменьшения накопления сухого вещества до плохого распределения метаболитов из стеблей и листьев в зерно, что приводит к уменьшению количества выполненных зерен в метелке, уменьшению массы зерновки и в конечном счете урожайности. Сортовое разнообразие риса включает в себя различные гены устойчивости к засухе. Цель исследований – оценка суходольных образцов и сортов риса по устойчивости к засухе в полевых условиях и наличию гена qDTY1.1 с использованием ДНК-маркера RM431. С помощью ПЦР-анализа было оценено 66 линий, гибридов и сортов риса, выращенных на периодически увлажняемом и постоянно залитом водой полях. В результате маркерного анализа было установлено наличие гена устойчивости к засухе qDTY1.1 у 22 сортов и образцов риса: Ан-Юн-Хо, Волгоградский, Сталинградский, Волгоградский х Атлант, Чан-Чунь-Ман и др. Наибольшее значение индекса засухоустойчивости (ИЗУ) оказалось у носителей аллеля qDTY1.1: маньчжурских суходольных сортов Ан-Юн-Хо (79,4 %), Чан-Чунь-Ман (88,5 %) и других образцов, полученных ранее от скрещивания сорта Чан-Чунь-Ман с урожайными сортами донской селекции: Раздольный, Боярин, Командор, Южанин, Кубояр. Выделившиеся образцы, несущие ген qDTY1.1, превышали остальные формы в среднем по урожайности в условиях засухи на 0,20 т/га, на контроле – 0,21 т/га, а по ИЗУ – на 3,9 %. В долгосрочной перспективе повышения засухоустойчивости риса необходимо выявлять и использовать другие QTL с большими и постоянными эффектами и ключевыми регуляторами реакции растений на стресс.</p></abstract><trans-abstract xml:lang="en"><p>Drought is the main stress factor when growing plants. The effects of drought on rice plants range from reduced dry matter accumulation to poor distribution of metabolites from stems and leaves into grain, resulting in reduced number of full grains per panicle, reduced grain weight and ultimately yield. Rice varietal diversity includes different drought tolerance genes. The purpose of the current research was to estimate upland rice samples and varieties for drought resistance under field conditions and the presence of the qDTY1.1 gene using the DNA marker RM431. PCR analysis was used to estimate 66 rice lines, hybrids and varieties grown in periodically wetted and constantly flooded fields. As a result of marker analysis, there has been identified the presence of the drought resistance gene qDTY1.1 in twenty-two rice varieties and samples such as ‘An-Yun-Ho’, ‘Volgogradsky’, ‘Stalingradsky’, ‘Volgogradsky x Atlant’, ‘Chan-Chun-Man’, etc. The highest value of the drought resistance index (DRI) was found in carriers of the qDTY1.1 allele, they are Manchurian upland varieties ‘An-Yun-Ho’ (79.4 %), ‘Chan-Chun-Man’ (88.5 %) and other samples developed earlier from crossing the variety ‘Chan-Chun-Man’ with productive varieties of Don selection ‘Razdolny’, ‘Boyarin’, ‘Komandor’, ‘Yuzhanin’, ‘Kuboyar’. The identified samples carrying the qDTY1.1 gene exceeded the other forms on average in terms of productivity under drought conditions by 0.20 t/ha, under the control by 0.21 t/ha, and under IZU by 3.9 %. In the long term of improving rice drought tolerance, it is necessary to identify and exploit other QTLs with large and consistent effects and key regulators of plant stress responses.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>рис</kwd><kwd>сорт</kwd><kwd>засухоустойчивость</kwd><kwd>ген</kwd><kwd>маркер</kwd><kwd>урожайность</kwd></kwd-group><kwd-group xml:lang="en"><kwd>rice</kwd><kwd>variety</kwd><kwd>drought resistance</kwd><kwd>gene</kwd><kwd>marker</kwd><kwd>productivity</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Dar M. H., Waza S. A., Shukla S., Zaidi N. W., Nayak S., Hossain M., Kumar A., Ismail A. B., Singh U. S. Drought tolerant rice for ensuring food security in eastern India // Sustainability. 2020. Vol. 12(6), 17 p. DOI: 10.3390/su12062214</mixed-citation><mixed-citation xml:lang="en">Dar M. H., Waza S. A., Shukla S., Zaidi N. 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